Single-cell genomics

C Paolillo, E Londin, P Fortina - Clinical chemistry, 2019 - academic.oup.com
BACKGROUND Single-cell genomics is an approach to investigate cell heterogeneity and to
identify new molecular features correlated with clinical outcomes. This approach allows …

Inferred allelic variants of immunoglobulin receptor genes: a system for their evaluation, documentation, and naming

M Ohlin, C Scheepers, M Corcoran, WD Lees… - Frontiers in …, 2019 - frontiersin.org
Immunoglobulins or antibodies are the main effector molecules of the B-cell lineage and are
encoded by hundreds of variable (V), diversity (D), and joining (J) germline genes, which …

Observed antibody space: a resource for data mining next-generation sequencing of antibody repertoires

A Kovaltsuk, J Leem, S Kelm, J Snowden… - The Journal of …, 2018 - journals.aai.org
Abs are immune system proteins that recognize noxious molecules for elimination. Their
sequence diversity and binding versatility have made Abs the primary class of …

High-quality library preparation for NGS-based immunoglobulin germline gene inference and repertoire expression analysis

N Vázquez Bernat, M Corcoran, U Hardt… - Frontiers in …, 2019 - frontiersin.org
Next generation sequencing (NGS) of immunoglobulin (Ig) repertoires (Rep-seq) enables
examination of the adaptive immune system at an unprecedented level. Applications include …

Rituximab-resistant splenic memory B cells and newly engaged naive B cells fuel relapses in patients with immune thrombocytopenia

E Crickx, P Chappert, A Sokal, S Weller… - Science translational …, 2021 - science.org
Rituximab (RTX), an antibody targeting CD20, is widely used as a first-line therapeutic
strategy in B cell–mediated autoimmune diseases. However, a large proportion of patients …

PD-1/PD-L1 immune checkpoint and p53 loss facilitate tumor progression in activated B-cell diffuse large B-cell lymphomas

M Pascual, M Mena-Varas, EF Robles… - Blood, The Journal …, 2019 - ashpublications.org
Refractory or relapsed diffuse large B-cell lymphoma (DLBCL) often associates with the
activated B-cell-like (ABC) subtype and genetic alterations that drive constitutive NF-κB …

[HTML][HTML] AIRR-C IG Reference Sets: curated sets of immunoglobulin heavy and light chain germline genes

AM Collins, M Ohlin, M Corcoran, JM Heather… - Frontiers in …, 2024 - frontiersin.org
Introduction Analysis of an individual's immunoglobulin (IG) gene repertoire requires the use
of high-quality germline gene reference sets. When sets only contain alleles supported by …

Polymorphisms in human immunoglobulin heavy chain variable genes and their upstream regions

I Mikocziova, M Gidoni, I Lindeman… - Nucleic Acids …, 2020 - academic.oup.com
Germline variations in immunoglobulin genes influence the repertoire of B cell receptors and
antibodies, and such polymorphisms may impact disease susceptibility. However, the …

Anchor Clustering for million-scale immune repertoire sequencing data

H Chang, DA Ashlock, SP Graether, SM Keller - BMC bioinformatics, 2024 - Springer
Background The clustering of immune repertoire data is challenging due to the
computational cost associated with a very large number of pairwise sequence comparisons …

Computational evaluation of B-cell clone sizes in bulk populations

AM Rosenfeld, W Meng, DY Chen, B Zhang… - Frontiers in …, 2018 - frontiersin.org
B cell clones expand and contract during adaptive immune responses and can persist or
grow uncontrollably in lymphoproliferative disorders. One way to monitor and track B cell …