Purification of human CD34+ CD90+ HSCs reduces target cell population and improves lentiviral transduction for gene therapy

S Radtke, D Pande, M Cui, AM Perez, YY Chan… - … Therapy Methods & …, 2020 - cell.com
Hematopoietic stem cell (HSC) gene therapy has the potential to cure many genetic,
malignant, and infectious diseases. We have shown in a nonhuman primate gene therapy …

High‐sensitivity flow cytometric assays: considerations for design control and analytical validation for identification of rare events

U Sommer, S Eck, L Marszalek… - Cytometry Part B …, 2021 - Wiley Online Library
The current consensus recommendation papers dealing with the unique requirements for
the analytical validation of assays performed by flow cytometry address the validation of …

Systematic enzyme mapping of cellular metabolism by phasor-analyzed label-free NAD (P) H fluorescence lifetime imaging

R Leben, M Köhler, H Radbruch, AE Hauser… - International Journal of …, 2019 - mdpi.com
In the past years, cellular metabolism of the immune system experienced a revival, as it has
become clear that it is not merely responsible for the cellular energy supply, but also impacts …

Endoglin-aptamer-functionalized liposome-equipped PD-1-silenced T cells enhance antitumoral immunotherapeutic effects

S Xie, X Hou, W Yang, W Shi, X Yang… - International Journal …, 2021 - Taylor & Francis
Background The broader application of adoptive cell therapy (ACT) in cancer
immunotherapies (particularly for solid tumors) has always been limited by the …

Assessment of gram-and viability-staining methods for quantifying bacterial community dynamics using flow cytometry

A Duquenoy, S Bellais, C Gasc, C Schwintner… - Frontiers in …, 2020 - frontiersin.org
Over the past years, gut microbiota became a major field of interest with increasing reports
suggesting its association with a large number of human diseases. In this context, there is a …

Solutions for shared resource lab remote quality control and instrument troubleshooting during a pandemic

DM Gravano, U Chakraborty, I Pesce… - Cytometry Part …, 2021 - Wiley Online Library
The COVID‐19 pandemic has dramatically affected shared resource lab (SRL) staff in‐
person availability at institutions globally. This article discusses the challenges of ensuring …

Establishing CD19 B-cell reference control materials for comparable and quantitative cytometric expression analysis

L Wang, R Bhardwaj, H Mostowski, PN Patrone… - PLoS …, 2021 - journals.plos.org
In the field of cell-based therapeutics, there is a great need for high-quality, robust, and
validated measurements for cell characterization. Flow cytometry has emerged as a critically …

Best practices for optimization and validation of flow cytometry‐based receptor occupancy assays

E Hilt, YS Sun, TW McCloskey, S Eck… - Cytometry Part B …, 2021 - Wiley Online Library
In the development of therapeutic compounds that bind cell surface molecules, it is critical to
demonstrate the extent to which the drug engages its target. For cell‐associated targets, flow …

Progress towards global standardization for quantitative flow cytometry

C Gonneau, L Wang, S Mitra-Kaushik, PC Trampont… - Bioanalysis, 2021 - Taylor & Francis
The power of flow cytometry stems from the ability to conduct multiparametric measurements
on individual cells with exquisite specificity and sensitivity. The application of this technology …

Cyt‐Geist: Current and future challenges in cytometry: Reports of the CYTO 2019 Conference Workshops

K Czechowska, J Lannigan, N Aghaeepour… - Cytometry Part …, 2019 - Wiley Online Library
IN MAY 2018, we initiated a new, unique project: the CYTO Conference workshop summary
report. The main motivation was to present to the reader key findings from the live …