Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …

Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …

Glycans modify lipids and proteins to mediate inter-and intramolecular interactions across all
domains of life. RNA is not thought to be a major target of glycosylation. Here, we challenge …

Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease with high resistance to
therapies. Inflammatory and immunomodulatory signals co-exist in the pancreatic tumour …

Mitochondria are key organelles for cellular energetics, metabolism, signaling, and quality
control and have been linked to various diseases. Different views exist on the composition of …

CRISPR gene editing holds great promise to modify DNA sequences in somatic cells to treat
disease. However, standard computational and biochemical methods to predict off-target …

Despite its success in achieving the long-term survival of 10–30% of treated individuals,
immune therapy is still ineffective for most patients with cancer,. Many efforts are therefore …

The NLRP3 inflammasome plays a central role in antimicrobial defense as well as in the
context of sterile inflammatory conditions. NLRP3 activity is governed by two independent …

Metabolic reprogramming is a hallmark of activated T cells. The switch from oxidative
phosphorylation to aerobic glycolysis provides energy and intermediary metabolites for the …

CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats-associated protein
9) shows the opportunity to treat a diverse array of untreated various genetic and …