Charged and polar groups, through forming ion pairs, hydrogen bonds, and other less
specific electrostatic interactions, impart important properties to proteins. Modulation of the …

Fluorescence microscopy provides an efficient and unique approach to study fixed and
living cells because of its versatility, specificity, and high sensitivity. Fluorescence …

Förster (or fluorescence) resonance energy transfer (FRET) and fluorescence lifetime
imaging (FLIM) have moved center stage and are increasingly forming part of multifaceted …

This article continues the series of Surveys and Summaries on restriction endonucleases
(REases) begun this year in Nucleic Acids Research. Here we discuss 'Type II'REases, the …

Pairs of Au nanoparticles have recently been proposed as “plasmon rulers” based on the
dependence of their light scattering on the interparticle distance. Preliminary work has …

DNA methylation is a key regulator of gene expression and changes in DNA methylation
occur early in tumorigenesis. Mutations in the de novo DNA methyltransferase gene …

Many genetic processes depend on proteins interacting with specific sequences on DNA.
Despite the large excess of nonspecific DNA in the cell, proteins can locate their targets …

In stationary-phase Escherichia coli, Dps (DNA-binding protein from starved cells) is the
most abundant protein component of the nucleoid. Dps compacts DNA into a dense complex …

The fundamental theory of Förster resonance energy transfer (FRET) was established in the
1940s. Its great power was only realized in the past 20 years after different techniques were …

A facile and universal aptamer-based label-free approach for selective and sensitive
fluorescence detection of proteins and small biomolecules by using the SYBR Green I (SGI) …