Chitinase from Thermomyces lanuginosus SSBP and its biotechnological applications
Chitinases are ubiquitous class of extracellular enzymes, which have gained attention in the
past few years due to their wide biotechnological applications. The effectiveness of …
past few years due to their wide biotechnological applications. The effectiveness of …
Structural characterization of MG and pre-MG states of proteins by MD simulations, NMR, and other techniques
Almost all proteins fold via a number of partially structured intermediates such as molten
globule (MG) and pre-molten globule states. Understanding the structure of these …
globule (MG) and pre-molten globule states. Understanding the structure of these …
Measuring Structural Changes in Cytochrome c under Crowded Conditions Using In Vitro and In Silico Approaches
It is known from in vitro studies that macromolecular crowding in the cell effects protein
structure, stability and function; but predictive studies are relatively unexplored. There are …
structure, stability and function; but predictive studies are relatively unexplored. There are …
Protein folding: Molecular dynamics simulations and in vitro studies for probing mechanism of urea-and guanidinium chloride-induced unfolding of horse cytochrome …
Urea-and guanidinium chloride (GdmCl)-induced denatured states of horse cytochrome-c
(cyt-c) are structurally identical. It is then expected that estimates of∆ G 0 N→ U (Gibbs free …
(cyt-c) are structurally identical. It is then expected that estimates of∆ G 0 N→ U (Gibbs free …
GdmCl-induced unfolding studies of human carbonic anhydrase IX: a combined spectroscopic and MD simulation approach
Carbonic anhydrase IX (CAIX) is a transmembrane glycoprotein, associated with tumor,
acidification which leads to the cancer, and is considered as a potential biomarker for …
acidification which leads to the cancer, and is considered as a potential biomarker for …
In vitro and in silico studies of urea-induced denaturation of yeast iso-1-cytochrome c and its deletants at pH 6.0 and 25 °C
Yeast iso-1-cytochrome c (y-cyt-c) has five extra residues at N-terminus in comparison to the
horse cytochrome c. These residues are numbered as–5 to–1. Here, these extra residues …
horse cytochrome c. These residues are numbered as–5 to–1. Here, these extra residues …
Identification of natural small molecule modulators of MurB from Salmonella enterica serovar Typhi Ty2 strain using computational and biophysical approaches
The increase of antibiotic‐resistant bacterial pathogens has created challenges in treatment
and warranted the design of antibiotics against comparatively less exploited targets. The …
and warranted the design of antibiotics against comparatively less exploited targets. The …
Effect of sequential deletion of extra N-terminal residues on the structure and stability of yeast iso-1-cytochrome-c
A sequence alignment of yeast cytochrome-c (y-cyt-c) with mammalian cyts-c shows that the
yeast protein has a five residue long N-terminal extension. A question arises: Does this N …
yeast protein has a five residue long N-terminal extension. A question arises: Does this N …
Estimation of thermodynamic stability of human carbonic anhydrase IX from urea-induced denaturation and MD simulation studies
Carbonic anhydrase IX (CAIX) is a transmembrane glycoprotein, overexpressed in cancer
cells under hypoxia condition. In cancerous cells, CAIX plays an important role to combat the …
cells under hypoxia condition. In cancerous cells, CAIX plays an important role to combat the …
Evidence of non-coincidence of normalized sigmoidal curves of two different structural properties for two-state protein folding/unfolding
In practice, the observation of non-coincidence of normalized sigmoidal transition curves
measured by two different structural properties constitutes a proof of existence of …
measured by two different structural properties constitutes a proof of existence of …