Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated
transposases have the potential to transform the technology landscape for kilobase-scale …

While genome-wide transposon mutagenesis screens have identified numerous essential
genes in the significant human pathogen Streptococcus pyogenes (group A Streptococcus …

The CRISPR-Cas system has enabled the development of sophisticated, multigene
metabolic engineering programs through the use of guide RNA-directed activation or …

Synthetic perturbation of gene expression is central to our ability to reliably uncover
genotype–phenotype relationships in microbes. Here, we present a novel transcription …

Deciphering gene function is fundamental to engineering of microbiology. The clustered
regularly interspaced short palindromic repeats (CRISPR) system has been adapted for …

Engineering metabolism to efficiently produce chemicals from multi-step pathways requires
optimizing multi-gene expression programs to achieve enzyme balance. CRISPR-Cas …

In the past decades, the broad selection of CRISPR-Cas systems has revolutionized
biotechnology by enabling multimodal genetic manipulation in diverse organisms. Rooted in …

Clustered regularly interspaced short palindromic repeats (CRISPR) has revolutionized the
field of genome editing. To circumvent the permanent modifications made by traditional …

Robust control over gene translation at arbitrary mRNA targets is an outstanding challenge
in microbial synthetic biology. The development of tools that can regulate translation will …