Development of biosensing platforms plays a key role in research settings for identification
of biomarkers and in clinical applications for diagnostics. Biosensors based on nucleic acids …

All soluble proteins populate conformational ensembles that together constitute the native
state. Their fluctuations in water are intrinsic thermodynamic phenomena, and the …

Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to
determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However …

A goal in the kinetic characterization of a macromolecular system is the description of its
slow relaxation processes via (i) identification of the structural changes involved in these …

Markov state models of molecular kinetics (MSMs), in which the long-time statistical
dynamics of a molecule is approximated by a Markov chain on a discrete partition of …

Single-molecule fluorescence spectroscopy and super-resolution microscopy are important
elements of the ongoing technical revolution to reveal biochemical and cellular processes in …

Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …

DNA polymerase θ (Polθ) plays a central role in a DNA double-strand break repair pathway
termed theta-mediated end joining (TMEJ). TMEJ functions by pairing short-sequence" …

The alternating-laser excitation (ALEX) scheme continues to expand the possibilities of
fluorescence-based assays to study biological entities and interactions. Especially the …

Single‐pair Förster resonance energy transfer (spFRET) experiments using single‐molecule
burst analysis on a confocal microscope are an ideal tool to measure inter‐and …