Hydroxyl radical protein footprinting (HRPF) coupled to mass spectrometry has been
successfully used to investigate a plethora of protein-related questions. The method, which …

Biotherapeutics and their biosimilar versions have been flourishing in the biopharmaceutical
market for several years. Structural and functional characterization is needed to achieve …

Hydroxyl radical protein footprinting (HRPF) is a powerful and flexible technique for probing
changes in protein topography. With the development of the fast photochemical oxidation of …

X-ray radiolytic labeling uses broadband X-rays for in situ hydroxyl radical labeling to map
protein interactions and conformation. High flux density beams are essential to overcome …

Protein posttranslational modifications (PTMs) are key modulators of protein structure and
function that often change in a dynamic fashion in response to cellular stimuli. Dynamic …

Unlike small molecule drugs, therapeutic proteins must maintain the proper higher-order
structure (HOS) in order to maintain safety and efficacy. Due to the sensitivity of many protein …

Commercial monoclonal antibodies are growing and important components of modern
therapies against a multitude of human diseases. Well-known high-resolution structural …

Hydroxyl radical protein footprinting (HRPF) using synchrotron X-ray radiation (XFP) and
mass spectrometry is a well-validated structural biology method that provides critical insights …

Hydroxyl radical protein footprinting (HRPF) using synchrotron radiation is a well-validated
method to assess protein structure in the native solution state. In this method, X-ray …

Fast photochemical oxidation of proteins (FPOP) is a hydroxyl radical footprinting approach
whereby radicals, produced by UV laser photolysis of hydrogen peroxide, induce oxidation …