The recent increase in genomic data is revealing an unexpected perspective of gene loss as
a pervasive source of genetic variation that can cause adaptive phenotypic diversity. This …

Genetic interactions identify combinations of genetic variants that impinge on phenotype.
With whole-genome sequence information available for thousands of individuals within a …

INTRODUCTION Genetic interactions occur when mutations in two or more genes combine
to generate an unexpected phenotype. An extreme negative or synthetic lethal genetic …

We developed a systematic approach to map human genetic networks by combinatorial
CRISPR–Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all …

INTRODUCTION Genetic interactions occur when mutations in different genes combine to
result in a phenotype that is different from expectation based on those of the individual …

In this essay, we revisit the status of yeast as a model system for biology. We first summarize
important contributions of yeast to eukaryotic biology that we anticipated in 1988 in our first …

The yeast deletion collections comprise> 21,000 mutant strains that carry precise start-to-
stop deletions of∼ 6000 open reading frames. This collection includes heterozygous and …

As cancer cell genomes are unveiled at a breathtaking pace, the genetic principles at play in
cancer are emerging in all their complexity, prompting the assessment of classical genetic …

Relocalization of proteins is a hallmark of the DNA damage response. We use high-
throughput microscopic screening of the yeast GFP fusion collection to develop a systems …

Drug synergy allows a therapeutic effect to be achieved with lower doses of component
drugs. Drug synergy can result when drugs target the products of genes that act in parallel …