The first-order rate constants for the hydrolysis of a phosphodiester bond under
physiological conditions have been estimated to be 6 X 10-9 and 6 X 10-10 min-1 for single …

MOLECULAR BIOLOGY OF THE LysR FAMILY OF TRANSCRIPTIONAL REGULATORS Page 1
Annu. Rev. Microbiol. 1993. 47:597-{J26 Copyright © 1993 by Annual Reviews Inc. All rights …

The gel electrophoresis mobility shift assay (EMSA) is used to detect protein complexes with
nucleic acids. It is the core technology underlying a wide range of qualitative and …

“Footprinting” refers to assays that examine ligand binding and conformational changes by
determining the solvent accessibility of the backbone, bases, or side chain structures of …

Hydroxyl radical protein footprinting (HRPF) coupled to mass spectrometry has been
successfully used to investigate a plethora of protein-related questions. The method, which …

C/EBP is a sequence-specific DNA binding protein that regulates gene expression in certain
mammalian cells. The region of the C/EBP polypeptide required for specific recognition of …

1. INTRODUCTION In eukaryotic cells, inheritable information is stored in a nucleoprotein
complex referred to as chromatin. 1 This genome architecture serves two key purposes. On …

Publisher Summary This chapter discusses DNA and RNA cleavage by metal complexes.
DNA and RNA cleavage, a very active field of research, has been developed in two main …

This review article focuses on the molecular aspects of DNA cleavage by synthetic chemical
nucleases (transition metal complexes endowed with redox properties and DNA affinity) and …

Tardigrades, also known as water bears, are animals that can survive extreme conditions.
The tardigrade Ramazzottius varieornatus contains a unique nuclear protein termed Dsup …