Thermostable enzymes as biocatalysts in the biofuel industry
Lignocellulose is the most abundant carbohydrate source in nature and represents an ideal
renewable energy source. Thermostable enzymes that hydrolyze lignocellulose to its …
renewable energy source. Thermostable enzymes that hydrolyze lignocellulose to its …
The crystal structures of Sinapis alba myrosinase and a covalent glycosyl–enzyme intermediate provide insights into the substrate recognition and active-site …
WP Burmeister, S Cottaz, H Driguez, R Iori, S Palmieri… - Structure, 1997 - cell.com
Background: Myrosinase is the enzyme responsible for the hydrolysis of a variety of plant
anionic 1-thio-β-D-glucosides called glucosinolates. Myrosinase and glucosinolates, which …
anionic 1-thio-β-D-glucosides called glucosinolates. Myrosinase and glucosinolates, which …
Crystal structure of the β-glycosidase from the hyperthermophilic archeon Sulfolobus solfataricus: resilience as a key factor in thermostability
CF Aguilar, I Sanderson, M Moracci… - Journal of molecular …, 1997 - Elsevier
Enzymes from hyperthermophilic organisms must operate at temperatures which rapidly
denature proteins from mesophiles. The structural basis of this thermostability is still poorly …
denature proteins from mesophiles. The structural basis of this thermostability is still poorly …
The mechanism of substrate (aglycone) specificity in β-glucosidases is revealed by crystal structures of mutant maize β-glucosidase-DIMBOA,-DIMBOAGlc, and …
M Czjzek, M Cicek, V Zamboni… - Proceedings of the …, 2000 - National Acad Sciences
The mechanism and the site of substrate (ie, aglycone) recognition and specificity were
investigated in maize β-glucosidase (Glu1) by x-ray crystallography by using crystals of a …
investigated in maize β-glucosidase (Glu1) by x-ray crystallography by using crystals of a …
Molecular mechanism of Aspergillus fumigatus biofilm disruption by fungal and bacterial glycoside hydrolases
F Le Mauff, NC Bamford, N Alnabelseya… - Journal of Biological …, 2019 - ASBMB
During infection, the fungal pathogen Aspergillus fumigatus forms biofilms that enhance its
resistance to antimicrobials and host defenses. An integral component of the biofilm matrix is …
resistance to antimicrobials and host defenses. An integral component of the biofilm matrix is …
Restoration of the Activity of Active-Site Mutants of the Hyperthermophilic β-Glycosidase from Sulfolobus solfataricus: Dependence of the Mechanism on the Action …
The β-glycosidase from the hyperthermophilic Archaeon Sulfolobus solfataricus hydrolyzes
β-glycosides following a retaining mechanism based upon the action of two amino acids …
β-glycosides following a retaining mechanism based upon the action of two amino acids …
Residue Effect-Guided Design: Engineering of S. Solfataricus β-Glycosidase to Enhance Its Thermostability and Bioproduction of Ginsenoside Compound K
β-Glycosidase from Sulfolobus solfataricus (SS-BGL) is a highly effective biocatalyst for the
synthesis of compound K (CK) from glycosylated protopanaxadiol ginsenosides. In order to …
synthesis of compound K (CK) from glycosylated protopanaxadiol ginsenosides. In order to …
Comparative Structural Analysis and Substrate Specificity Engineering of the Hyperthermostable β-Glucosidase CelB from Pyrococcus furiosus
The substrate specificity of the β-glucosidase (CelB) from the hyperthermophilic archaeon
Pyrococcus furiosus, a family 1 glycosyl hydrolase, has been studied at a molecular level …
Pyrococcus furiosus, a family 1 glycosyl hydrolase, has been studied at a molecular level …
Substrate specificity and transglycosylation catalyzed by a thermostable β-glucosidase from marine hyperthermophile Thermotoga neapolitana
TH Park, KW Choi, CS Park, SB Lee, HY Kang… - Applied microbiology …, 2005 - Springer
The gene encoding β-glucosidase of the marine hyperthermophilic eubacterium
Thermotoga neapolitana (bglA) was subcloned and expressed in Escherichia coli. The …
Thermotoga neapolitana (bglA) was subcloned and expressed in Escherichia coli. The …
Identification and molecular characterization of the first α-xylosidase from an archaeon
We here report the first molecular characterization of an α-xylosidase (XylS) from an
Archaeon. Sulfolobus solfataricus is able to grow at temperatures higher than 80° C on …
Archaeon. Sulfolobus solfataricus is able to grow at temperatures higher than 80° C on …