Gene therapy: Comprehensive overview and therapeutic applications

N Sayed, P Allawadhi, A Khurana, V Singh, U Navik… - Life sciences, 2022 - Elsevier
Gene therapy is the product of man's quest to eliminate diseases. Gene therapy has three
facets namely, gene silencing using siRNA, shRNA and miRNA, gene replacement where …

Next-generation sequencing for confronting virus pandemics

J Quer, S Colomer-Castell, C Campos, C Andrés… - Viruses, 2022 - mdpi.com
Virus pandemics have happened, are happening and will happen again. In recent decades,
the rate of zoonotic viral spillover into humans has accelerated, mirroring the expansion of …

The role of hepatitis B virus genome variations in HBV-related HCC: effects on host signaling pathways

S Shoraka, SM Hosseinian, A Hasibi… - Frontiers in …, 2023 - frontiersin.org
Hepatocellular carcinoma (HCC) is a significant global health issue, with a high prevalence
in many regions. There are variations in the etiology of HCC in different regions, but most …

The impact of HBV quasispecies features on immune status in HBsAg+/HBsAb+ patients with HBV genotype C using next-generation sequencing

Y Wang, X Xiao, S Chen, C Huang, J Zhou… - Frontiers in …, 2021 - frontiersin.org
Background This study aimed to explore the molecular mechanism of the coexistence of
hepatitis B surface antigen (HBsAg) and hepatitis B surface antibody (HBsAb) serological …

Inspecting the ribozyme region of hepatitis delta virus genotype 1: conservation and variability

B Pacin-Ruiz, MF Cortese, D Tabernero, S Sopena… - Viruses, 2022 - mdpi.com
The hepatitis delta virus (HDV) genome has an autocatalytic region called the ribozyme,
which is essential for viral replication. The aim of this study was to use next-generation …

Genotyping Hepatitis B virus by Next-Generation Sequencing: Detection of Mixed Infections and Analysis of Sequence Conservation

E Dopico, M Vila, D Tabernero, J Gregori… - International Journal of …, 2024 - mdpi.com
Our aim was to develop an accurate, highly sensitive method for HBV genotype
determination and detection of genotype mixtures. We examined the preS and 5′ end of …

Hepatitis B Virus Variants with Multiple Insertions and/or Deletions in the X Open Reading Frame 3′ End: Common Members of Viral Quasispecies in Chronic …

S García-García, A Caballero-Garralda, D Tabernero… - Biomedicines, 2022 - mdpi.com
Deletions in the 3′ end region of the hepatitis B virus (HBV) X open reading frame (HBX)
may affect the core promoter (Cp) and have been frequently associated with hepatocellular …

[PDF][PDF] Next-generation sequencing for confronting virus pandemics. Viruses 2022; 14: 600

J Quer, S Colomer-Castell, C Campos, C Andrés… - 2022 - academia.edu
Virus pandemics have happened, are happening and will happen again. In recent decades,
the rate of zoonotic viral spillover into humans has accelerated, mirroring the expansion of …

Comparative Metagenomics to Study the Impact of Soil Salinity on Microbial Diversity in Al-Madinah, KSA

MS Waznah, HM Albasri, HA Albahri - 2023 - researchsquare.com
The chemical properties and fertility of soil are greatly influenced by soil microbes, which are
essential to the biogeochemical cycle. Analyzing the microbial structure of soil is important …

Studying Hepatitis B and Hepatitis Delta viruses quasispecies by Next-generation sequencing: Conservation, variability, and interaction

B Pacín Ruiz - 2023 - tdx.cat
The Hepatitis B virus (HBV) affects around 296 million people worldwide. Of them, more than
5% are coinfected with Hepatitis delta virus (HDV), causing the most severe viral hepatitis …