Microalgae-based biotechnological sequestration of carbon dioxide for net zero emissions

Z Ma, WY Cheah, IS Ng, JS Chang, M Zhao… - Trends in …, 2022 - cell.com
Excessive carbon dioxide (CO 2) emissions into the atmosphere have become a dire threat
to the human race and environmental sustainability. The ultimate goal of net zero emissions …

Application of different types of CRISPR/Cas-based systems in bacteria

Z Liu, H Dong, Y Cui, L Cong, D Zhang - Microbial cell factories, 2020 - Springer
As important genome editing tools, CRISPR/Cas systems, especially those based on type II
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …

Cas9 off-target binding to the promoter of bacterial genes leads to silencing and toxicity

W Rostain, T Grebert, D Vyhovskyi… - Nucleic Acids …, 2023 - academic.oup.com
Genetic tools derived from the Cas9 RNA-guided nuclease are providing essential
capabilities to study and engineer bacteria. While the importance of off-target effects was …

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

P Calero, PI Nikel - Microbial Biotechnology, 2019 - Wiley Online Library
The last few years have witnessed an unprecedented increase in the number of novel
bacterial species that hold potential to be used for metabolic engineering. Historically …

Development of a confinable gene drive system in the human disease vector Aedes aegypti

M Li, T Yang, NP Kandul, M Bui, S Gamez, R Raban… - Elife, 2020 - elifesciences.org
Aedes aegypti is the principal mosquito vector for many arboviruses that increasingly infect
millions of people every year. With an escalating burden of infections and the relative failure …

Cpf1 is a versatile tool for CRISPR genome editing across diverse species of cyanobacteria

J Ungerer, HB Pakrasi - Scientific reports, 2016 - nature.com
Cyanobacteria are the ideal organisms for the production of a wide range of bioproducts as
they can convert CO2 directly into the desired end product using solar energy. Unfortunately …

Genetic engineering of microalgae for enhanced biorefinery capabilities

M Fayyaz, KW Chew, PL Show, TC Ling, IS Ng… - Biotechnology …, 2020 - Elsevier
Microalgae-based bioproducts are in limelight because of their promising future, novel
characteristics, the current situation of population needs, and rising prices of rapidly …

New applications of synthetic biology tools for cyanobacterial metabolic engineering

M Santos-Merino, AK Singh, DC Ducat - Frontiers in bioengineering …, 2019 - frontiersin.org
Cyanobacteria are promising microorganisms for sustainable biotechnologies, yet unlocking
their potential requires radical re-engineering and application of cutting-edge synthetic …

CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production

H Li, CR Shen, CH Huang, LY Sung, MY Wu… - Metabolic …, 2016 - Elsevier
Cyanobacteria hold promise as a cell factory for producing biofuels and bio-derived
chemicals, but genome engineering of cyanobacteria such as Synechococcus elongatus …

Recent developments on genetic engineering of microalgae for biofuels and bio‐based chemicals

IS Ng, SI Tan, PH Kao, YK Chang… - Biotechnology …, 2017 - Wiley Online Library
Microalgae serve as a promising source for the production of biofuels and bio‐based
chemicals. They are superior to terrestrial plants as feedstock in many aspects and their …