Small-molecule fluorescent probes for live-cell super-resolution microscopy
Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …
cellular structures at the nanometer scale. Employing these techniques in living cells has …
Cellular incorporation of unnatural amino acids and bioorthogonal labeling of proteins
In the past 15 years, there have been rapid developments in the ability to cotranslationally
incorporate unnatural amino acids into proteins produced in cells, both at defined sites via …
incorporate unnatural amino acids into proteins produced in cells, both at defined sites via …
Functionalizing nanoparticles with biological molecules: developing chemistries that facilitate nanotechnology
KE Sapsford, WR Algar, L Berti, KB Gemmill… - Chemical …, 2013 - ACS Publications
Bionanotechnology has now become firmly established in its own right as one of the
principle and focused subdisciplines within nanotechnology.(1-10) Bionanotechnology can …
principle and focused subdisciplines within nanotechnology.(1-10) Bionanotechnology can …
Bioorthogonal chemistry: fishing for selectivity in a sea of functionality
EM Sletten, CR Bertozzi - Angewandte Chemie International …, 2009 - Wiley Online Library
The study of biomolecules in their native environments is a challenging task because of the
vast complexity of cellular systems. Technologies developed in the last few years for the …
vast complexity of cellular systems. Technologies developed in the last few years for the …
Maleimide–thiol adducts stabilized through stretching
Maleimide–thiol reactions are widely used to produce protein–polymer conjugates for
therapeutics. However, maleimide–thiol adducts are unstable in vivo or in the presence of …
therapeutics. However, maleimide–thiol adducts are unstable in vivo or in the presence of …
Chemistry in living systems
JA Prescher, CR Bertozzi - Nature chemical biology, 2005 - nature.com
Dissecting complex cellular processes requires the ability to track biomolecules as they
function within their native habitat. Although genetically encoded tags such as GFP are …
function within their native habitat. Although genetically encoded tags such as GFP are …
The phosphopantetheinyl transferases: catalysis of a post-translational modification crucial for life
Covering: up to 2013 Although holo-acyl carrier protein synthase, AcpS, a
phosphopantetheinyl transferase (PPTase), was characterized in the 1960s, it was not until …
phosphopantetheinyl transferase (PPTase), was characterized in the 1960s, it was not until …
Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal reaction
The site-specific incorporation of bioorthogonal groups via genetic code expansion provides
a powerful general strategy for site-specifically labelling proteins with any probe. However …
a powerful general strategy for site-specifically labelling proteins with any probe. However …
Polymerization in living organisms
Vital biomacromolecules, such as RNA, DNA, polysaccharides and proteins, are
synthesized inside cells via the polymerization of small biomolecules to support and multiply …
synthesized inside cells via the polymerization of small biomolecules to support and multiply …
Engineering live cell surfaces with functional polymers via cytocompatible controlled radical polymerization
The capability to graft synthetic polymers onto the surfaces of live cells offers the potential to
manipulate and control their phenotype and underlying cellular processes. Conventional …
manipulate and control their phenotype and underlying cellular processes. Conventional …