Super-resolution fluorescence microscopy is a powerful tool to visualize biomolecules and
cellular structures at the nanometer scale. Employing these techniques in living cells has …

In the past 15 years, there have been rapid developments in the ability to cotranslationally
incorporate unnatural amino acids into proteins produced in cells, both at defined sites via …

Bionanotechnology has now become firmly established in its own right as one of the
principle and focused subdisciplines within nanotechnology.(1-10) Bionanotechnology can …

The study of biomolecules in their native environments is a challenging task because of the
vast complexity of cellular systems. Technologies developed in the last few years for the …

Maleimide–thiol reactions are widely used to produce protein–polymer conjugates for
therapeutics. However, maleimide–thiol adducts are unstable in vivo or in the presence of …

Dissecting complex cellular processes requires the ability to track biomolecules as they
function within their native habitat. Although genetically encoded tags such as GFP are …

Covering: up to 2013 Although holo-acyl carrier protein synthase, AcpS, a
phosphopantetheinyl transferase (PPTase), was characterized in the 1960s, it was not until …

The site-specific incorporation of bioorthogonal groups via genetic code expansion provides
a powerful general strategy for site-specifically labelling proteins with any probe. However …

Vital biomacromolecules, such as RNA, DNA, polysaccharides and proteins, are
synthesized inside cells via the polymerization of small biomolecules to support and multiply …

The capability to graft synthetic polymers onto the surfaces of live cells offers the potential to
manipulate and control their phenotype and underlying cellular processes. Conventional …