Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Fluorescence lifetime techniques in medical applications

L Marcu - Annals of biomedical engineering, 2012 - Springer
This article presents an overview of time-resolved (lifetime) fluorescence techniques used in
biomedical diagnostics. In particular, we review the development of time-resolved …

Generative adversarial network enables rapid and robust fluorescence lifetime image analysis in live cells

YI Chen, YJ Chang, SC Liao, TD Nguyen… - Communications …, 2022 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool to quantify molecular
compositions and study molecular states in complex cellular environment as the lifetime …

Fluorescence lifetime imaging microscopy for brain tumor image-guided surgery

Y Sun, N Hatami, M Yee, J Phipps… - … of biomedical optics, 2010 - spiedigitallibrary.org
We demonstrate for the first time the application of an endoscopic fluorescence lifetime
imaging microscopy (FLIM) system to the intraoperative diagnosis of glioblastoma …

A novel method for fast and robust estimation of fluorescence decay dynamics using constrained least-squares deconvolution with Laguerre expansion

J Liu, Y Sun, J Qi, L Marcu - Physics in Medicine & Biology, 2012 - iopscience.iop.org
We report a novel method for estimating fluorescence impulse response function (fIRF) from
noise-corrupted time-domain fluorescence measurements of biological tissue. This method …

Fluorescence lifetime spectroscopy for guided therapy of brain tumors

PV Butte, AN Mamelak, M Nuno, SI Bannykh, KL Black… - Neuroimage, 2011 - Elsevier
This study evaluates the potential of time-resolved laser induced fluorescence spectroscopy
(TR-LIFS) as intra-operative tool for the delineation of brain tumor from normal brain. Forty …

Intraoperative delineation of primary brain tumors using time-resolved fluorescence spectroscopy

PV Butte, Q Fang, JA Jo, WH Yong… - … of biomedical optics, 2010 - spiedigitallibrary.org
The goal of this study is to determine the potential of time-resolved laser-induced
fluorescence spectroscopy (TR-LIFS) as an adjunctive tool for delineation of brain tumor …

[PDF][PDF] A plasmonic platform with disordered array of metal nanoparticles for three-order enhanced upconversion luminescence and highly sensitive near-infrared …

SJ Kwon, GY Lee, K Jung, HS Jang, JS Park, H Ju… - Adv. Mater, 2016 - researchgate.net
Figure S1. 2D monolayer of triangular close-packed uniform UCNPs. Figure S2.
Optimization of SiO2 dielectric layer for the strongest UCL. Figure S3. Size distributions of …

Fluorescence Lifetime Spectroscopy of Glioblastoma Multiforme

L Marcu, JA Jo, PV Butte, WH Yong… - Photochemistry and …, 2004 - Wiley Online Library
Fluorescence spectroscopy of the endogenous emission of brain tumors has been
researched as a potentially important method for the intraoperative localization of brain …

Detection of rupture-prone atherosclerotic plaques by time-resolved laser-induced fluorescence spectroscopy

L Marcu, JA Jo, Q Fang, T Papaioannou, T Reil… - Atherosclerosis, 2009 - Elsevier
OBJECTIVE: Plaque with dense inflammatory cells, including macrophages, thin fibrous cap
and superficial necrotic/lipid core is thought to be prone-to-rupture. We report a time …