Synthetic and editing mechanisms of aminoacyl-tRNA synthetases

JJ Perona, I Gruic-Sovulj - Aminoacyl-tRNA synthetases in biology and …, 2014 - Springer
Aminoacyl-tRNA synthetases (aaRS) ensure the faithful transmission of genetic information
in all living cells. The 24 known aaRS families are divided into 2 structurally distinct classes …

Aminoacyl-tRNA synthetases in the bacterial world

R Giegé, M Springer - EcoSal Plus, 2016 - Am Soc Microbiol
Aminoacyl-tRNA synthetases (aaRSs) are modular enzymes globally conserved in the three
kingdoms of life. All catalyze the same two-step reaction, ie, the attachment of a …

Insights into the mechanism of tryptophan fluorescence quenching due to synthetic crowding agents: A combined experimental and computational study

CJ Fossum, BOV Johnson, ST Golde, AJ Kielman… - ACS …, 2023 - ACS Publications
Intrinsic tryptophan fluorescence spectroscopy is an important tool for examining the effects
of molecular crowding and confinement on the structure, dynamics, and function of proteins …

MD simulations of tRNA and aminoacyl-tRNA synthetases: dynamics, folding, binding, and allostery

R Li, LM Macnamara, JD Leuchter… - International Journal of …, 2015 - mdpi.com
While tRNA and aminoacyl-tRNA synthetases are classes of biomolecules that have been
extensively studied for decades, the finer details of how they carry out their fundamental …

Twenty years of exceptional success: The molecular education and research consortium in undergraduate computational chemistry (MERCURY)

GC Shields - International Journal of Quantum Chemistry, 2020 - Wiley Online Library
The molecular education and research consortium in undergraduate computational
chemistry (MERCURY) consortium, established in 2000, has contributed greatly to the …

Escherichia coli alanyl-tRNA synthetase maintains proofreading activity and translational accuracy under oxidative stress

A Kavoor, P Kelly, M Ibba - Journal of Biological Chemistry, 2022 - ASBMB
Aminoacyl-tRNA synthetases (aaRSs) are enzymes that synthesize aminoacyl-tRNAs to
facilitate translation of the genetic code. Quality control by aaRS proofreading and other …

Crowder-induced conformational ensemble shift in Escherichia coli Prolyl-tRNA synthetase

LM Adams, RJ Andrews, QH Hu, HL Schmit, S Hati… - Biophysical …, 2019 - cell.com
The effect of molecular crowding on the structure and function of Escherichia coli prolyl-
transfer RNA synthetase (Ec ProRS), a member of the aminoacyl-transfer RNA synthetase …

Polyethylene Glycol Impacts Conformation and Dynamics of Escherichia coli Prolyl-tRNA Synthetase Via Crowding and Confinement Effects

J Liebau, BF Laatsch, J Rusnak, K Gunderson… - Biochemistry, 2024 - ACS Publications
Polyethylene glycol (PEG) is a flexible, nontoxic polymer commonly used in biological and
medical research, and it is generally regarded as biologically inert. PEG molecules of …

Incorporating modeling and simulations in undergraduate biophysical chemistry course to promote understanding of structure‐dynamics‐function relationships in …

S Hati, S Bhattacharyya - Biochemistry and Molecular Biology …, 2016 - Wiley Online Library
A project‐based biophysical chemistry laboratory course, which is offered to the
biochemistry and molecular biology majors in their senior year, is described. In this course …

Cyclic Changes in Active Site Polarization and Dynamics Drive the “Ping-pong” Kinetics in NRH: Quinone Oxidoreductase 2: An Insight from QM/MM Simulations

CR Reinhardt, QH Hu, CG Bresnahan, S Hati… - ACS …, 2018 - ACS Publications
Quinone reductases belong to the family of flavin-dependent oxidoreductases. With the
redox active cofactor, flavin adenine dinucleotide, quinone reductases are known to utilize a …