Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal
amplification technique, operating at 37–42° C, with minimal sample preparation and …

BACKGROUND First introduced in 2006, recombinase polymerase amplification (RPA) has
stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them …

Isothermal molecular diagnostics are bridging the technology gap between traditional
diagnostics and polymerase chain reaction-based methods. These new techniques enable …

Recombinase polymerase amplification (RPA) technology relies on three major proteins,
recombinase proteins, single-strand binding proteins, and polymerases, to specifically …

Background Accurate diagnosis of urogenital schistosomiasis is vital for surveillance/control
programs. Amplification of schistosome DNA in urine by PCR is sensitive and specific but …

Background One of the key barriers preventing rapid diagnosis of leptospirosis is the lack of
available sensitive point-of-care testing. This study aimed to develop and validate a …

Background Plant viruses in the genus Begomovirus, family Geminiviridae often cause
substantial crop losses. These viruses have been emerging in many locations throughout …

Schistosomiasis is a debilitating disease affecting over 200 million people, with the highest
burden of morbidity and mortality in African countries. Despite its huge impact on the health …

Multiplexed isothermal amplification and detection of nucleic acid sequences and
biomarkers is of increasing importance in diverse areas including advanced diagnostics …

Background Accurate diagnosis of urogenital schistosomiasis is vital for surveillance and
control programmes. While a number of diagnostic techniques are available there is a need …