The effect of macromolecular crowding on mobility of biomolecules, association kinetics, and gene expression in living cells

M Tabaka, T Kalwarczyk, J Szymanski, S Hou… - Frontiers in …, 2014 - frontiersin.org
We discuss a quantitative influence of macromolecular crowding on biological processes:
motion, bimolecular reactions, and gene expression in prokaryotic and eukaryotic cells. We …

Polymers at interfaces and the interactions in colloidal dispersions

A Vrij - Pure and Applied Chemistry, 1976 - degruyter.com
Solutions of different polymers in the same solvent are incompatible as a rule and show
phase separation when they are mixed. H incompatibility is also to be observed in systems …

Extracellular fluid viscosity enhances liver cancer cell mechanosensing and migration

J Gonzalez-Molina, X Zhang, M Borghesan… - Biomaterials, 2018 - Elsevier
The extracellular fluid (ECF) is a crowded environment containing macromolecules that
determine its characteristic density, osmotic pressure, and viscosity, which greatly differ …

Crossover regime for the diffusion of nanoparticles in polyethylene glycol solutions: influence of the depletion layer

N Ziębacz, SA Wieczorek, T Kalwarczyk, M Fiałkowski… - Soft Matter, 2011 - pubs.rsc.org
The viscosity in soft matter systems is a scale dependent quantity. In polymer solutions the
viscosity of nanoprobes of size R approaches the macroscopic viscosity when R exceeds …

Tuning the catalytic properties of P22 nanoreactors through compositional control

J Sharma, T Douglas - Nanoscale, 2020 - pubs.rsc.org
Enzymes are biomacromolecular protein catalysts that are widely used in a plethora of
industrial-scale applications due to their high selectivity, efficiency and ability to work under …

Marangoni Droplets of Dextran in PEG Solution and Its Motile Change Due to Coil–Globule Transition of Coexisting DNA

T Furuki, H Sakuta, N Yanagisawa… - … Applied Materials & …, 2024 - ACS Publications
Motile droplets using Marangoni convection are attracting attention for their potential as cell-
mimicking small robots. However, the motion of droplets relative to the internal and external …

Effect of crowding by Dextrans in enzymatic reactions

I Pastor, L Pitulice, C Balcells, E Vilaseca… - Biophysical …, 2014 - Elsevier
The interior of the living cell is highly concentrated and structured with molecules that have
different shapes and sizes. Almost all experimental biochemical data have been obtained …

Macromolecular Crowding Effect upon in Vitro Enzyme Kinetics: Mixed Activation–Diffusion Control of the Oxidation of NADH by Pyruvate Catalyzed by Lactate …

C Balcells, I Pastor, E Vilaseca… - The Journal of …, 2014 - ACS Publications
Enzyme kinetics studies have been usually designed as dilute solution experiments, which
differ substantially from in vivo conditions. However, cell cytosol is crowded with a high …

TPE based aggregation induced emission fluorescent sensors for viscosity of liquid and mechanical properties of hydrogel

N Wang, H Yao, Q Tao, J Sun, H Ma, Y Wang… - Chinese Chemical …, 2022 - Elsevier
ABSTRACT Two amphiphilic TPE E/Z isomers with aggregation induced emission (AIE)
property have been synthesized and characterized. The logarithmic fluorescent intensity of …

Small crowders slow down kinesin-1 stepping by hindering motor domain diffusion

K Sozański, F Ruhnow, A Wiśniewska, M Tabaka… - Physical review …, 2015 - APS
The dimeric motor protein kinesin-1 moves processively along microtubules against forces
of up to 7 pN. However, the mechanism of force generation is still debated. Here, we point to …