Best practices in metabarcoding of fungi: from experimental design to results

L Tedersoo, M Bahram, L Zinger, RH Nilsson… - Molecular …, 2022 - Wiley Online Library
The development of high‐throughput sequencing (HTS) technologies has greatly improved
our capacity to identify fungi and unveil their ecological roles across a variety of ecosystems …

Current and emerging trends in techniques for plant pathogen detection

M Venbrux, S Crauwels, H Rediers - Frontiers in Plant Science, 2023 - frontiersin.org
Plant pathogenic microorganisms cause substantial yield losses in several economically
important crops, resulting in economic and social adversity. The spread of such plant …

Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?

R Lücking, MC Aime, B Robbertse, AN Miller… - IMA fungus, 2020 - Springer
True fungi (Fungi) and fungus-like organisms (eg Mycetozoa, Oomycota) constitute the
second largest group of organisms based on global richness estimates, with around 3 …

Primer, pipelines, parameters: issues in 16S rRNA gene sequencing

I Abellan-Schneyder, MS Matchado, S Reitmeier… - Msphere, 2021 - Am Soc Microbiol
Short-amplicon 16S rRNA gene sequencing is currently the method of choice for studies
investigating microbiomes. However, comparative studies on differences in procedures are …

Perspectives and benefits of high-throughput long-read sequencing in microbial ecology

L Tedersoo, M Albertsen, S Anslan… - Applied and …, 2021 - Am Soc Microbiol
Short-read, high-throughput sequencing (HTS) methods have yielded numerous important
insights into microbial ecology and function. Yet, in many instances short-read HTS …

The role of gut mycobiome in health and diseases

L Zhang, H Zhan, W Xu, S Yan… - Therapeutic advances in …, 2021 - journals.sagepub.com
The gut microbiome comprised of microbes from multiple kingdoms, including bacteria,
fungi, and viruses. Emerging evidence suggests that the intestinal fungi (the gut …

Identifying the 'unidentified'fungi: a global-scale long-read third-generation sequencing approach

L Tedersoo, S Anslan, M Bahram, U Kõljalg… - Fungal Diversity, 2020 - Springer
Molecular identification methods, in particular high-throughput sequencing tools, have
greatly improved our knowledge about fungal diversity and biogeography, but many of the …

[HTML][HTML] Comparing the significance of the utilization of next generation and third generation sequencing technologies in microbial metagenomics

I Akacin, Ş Ersoy, O Doluca, M Güngörmüşler - Microbiological Research, 2022 - Elsevier
Since the exploration of sequencing began in 2005, third and next-generation sequencing
(TGS and NGS) technologies have fundamentally changed metagenomics research. These …

[HTML][HTML] Applications of environmental DNA (eDNA) in agricultural systems: Current uses, limitations and future prospects

JH Kestel, DL Field, PW Bateman, NE White… - Science of the Total …, 2022 - Elsevier
Global food production, food supply chains and food security are increasingly stressed by
human population growth and loss of arable land, becoming more vulnerable to …

[HTML][HTML] Diversity, detection and exploitation: Linking soil fungi and plant disease

A Bollmann-Giolai, JG Malone, S Arora - Current Opinion in Microbiology, 2022 - Elsevier
Highlights•Plant-associated fungi in the rhizosphere are incredibly diverse.•High plant
diversity promotes fungal diversity and can suppress soil fungal pathogens.•Many …