Highlights•Methyl-specific labeling for solution NMR studies of proteins above 100
kDa.•Specific labeling of any combination of methyl groups in protein overexpressed in E …

Membrane proteins are still heavily under-represented in the protein data bank (PDB),
owing to multiple bottlenecks. The typical low abundance of membrane proteins in their …

Nuclear magnetic resonance (NMR) is widely used across many fields of science because
of the rich data it produces, and some of the most valuable data come from studies of …

Determination of structure of integral membrane proteins, especially in their native
environment, is a formidable challenge in structural biology. Here we demonstrate that …

Gloeobacter rhodopsin (GR) is a cyanobacterial proton pump which can be potentially
applied to optogenetics. We solved the crystal structure of GR and found that it has overall …

Membrane-protein NMR occupies a unique niche for determining structures, assessing
dynamics, examining folding, and studying the binding of lipids, ligands and drugs to …

Proteorhodopsins are the most abundant retinal based photoreceptors and their
phototrophic function might be relevant in marine ecosystems. Here, we describe their …

Proteorhodopsins (PRs), members of the microbial rhodopsin superfamily of seven-
transmembrane-helix proteins that use retinal chromophores, comprise the largest subfamily …

Membrane proteins frequently assemble into higher order homo-or hetero-oligomers within
their natural lipid environment. This complex formation can modulate their folding, activity as …

The determination of membrane protein (MP) structures has always trailed that of soluble
proteins due to difficulties in their overexpression, reconstitution into membrane mimetics …