Protein kinases play a predominant regulatory role in nearly every aspect of cell biology and
they can modify the function of a protein in almost every conceivable way. Protein …

Use of optical traps for the manipulation of biological particles was recently proposed, and
initial observations of laser trapping of bacteria and viruses with visible argon-laser light …

1 A glycogen synthase kinase was partially purified from rabbit skeletal muscle by
precipitation with ammonium sulphate, chromatography on DEAE‐cellulose and …

Wnt ligands working through Frizzled receptors have a differential ability to stimulate release
of intracellular calcium (Ca 2+) and activation of protein kinase C (PKC). Since targets of this …

Protein tyrosine kinases (PTKs) play a crucial role in many cell regulatory processes. It is
therefore not surprising to see that functional perturbation of PTKs results in many diseases …

Protein kinase activity results in the incorporation of radiolabeled phosphate from [γ-32 P]
ATP into a peptide or protein substrate. The measurement of the amount of radioactivity …

Background: The sarcoplasmic reticulum (SR) Ca2+-ATPase 2 (SERCA2) mediates Ca2+
reuptake into SR and thereby promotes cardiomyocyte relaxation, whereas the ryanodine …

Publisher Summary This chapter discusses the assays of the protein kinase. The
radioisotopic method for cAMP-dependent protein kinase can be used with tissue …

Analogues of staurosporine were synthesized and their ability to inhibit protein kinases was
examined. Staurosporine is a potent but non‐selective inhibitor of in vitro protein kinase C …

It has been difficult to establish whether cyclic AMP-mediated protein phosphorylation in
nerve cells plays a specific role in synaptic transmission. This difficulty can be overcome in …