The spliceosome removes introns from messenger RNA precursors (pre-mRNA). Decades
of biochemistry and genetics combined with recent structural studies of the spliceosome …

Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

Modeling flexible macromolecules is one of the foremost challenges in single-particle
cryogenic-electron microscopy (cryo-EM), with the potential to illuminate fundamental …

Recent advances in the field of electron cryomicroscopy (cryo-EM) have resulted in a rapidly
increasing number of atomic models of biomacromolecules that have been solved using this …

Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical
technique that can be used to determine the structure of biological macromolecules and …

By reaching near-atomic resolution for a wide range of specimens, single-particle cryo-EM
structure determination is transforming structural biology. However, the necessary …

Precursor messenger RNA (pre-mRNA) splicing is an essential step in the flow of
information from DNA to protein in all eukaryotes. Research over the past four decades has …

Recent studies have highlighted that splicing patterns are frequently altered in cancer and
that mutations in genes encoding spliceosomal proteins, as well as mutations affecting the …

Several macromolecular machines collaborate to produce eukaryotic messenger RNA. RNA
polymerase II (Pol II) translocates along genes that are up to millions of base pairs in length …

INTRODUCTION RNA cleavage and phosphorylation-dephosphorylation of the RNA
polymerase II (Pol II) C-terminal domain (CTD) are two regulatory mechanisms of …