Abstract Two‐dimensional gel electrophoresis (2‐DE) with immobilized pH gradients (IPGs)
combined with protein identification by mass spectrometry (MS) is currently the workhorse …

Two-dimensional gel electrophoresis has been instrumental in the birth and developments
of proteomics, although it is no longer the exclusive separation tool used in the field of …

We describe an automated method for shotgun proteomics named multidimensional protein
identification technology (MudPIT), which combines multidimensional liquid chromatography …

Proteome analysis implies the ability to separate proteins as a first step prior to
characterization. Thus, the overall performance of the analysis strongly depends on the …

Coronary artery thrombosis is often initiated by abrupt disruption of the atherosclerotic
plaque and activation of platelets on the subendothelial layers in the disrupted plaque. The …

Outer membrane proteins (OMPs) of Gram‐negative bacteria are key molecules that
interface the cell with the environment. Traditional biochemical and genetic approaches …

The complete set of nuclear genes that encode proteins targeted to mitochondria in plants is
currently undefined and thus the full range of mitochondrial functions in plants is unknown …

Reverse phase protein array (RPPA) technology introduced a miniaturized “antigen-down”
or “dot-blot” immunoassay suitable for quantifying the relative, semi-quantitative or …

Complex I purified from bovine heart mitochondria is a multisubunit membrane-bound
assembly. In the past, seven of its subunits were shown to be products of the mitochondrial …

Sample preparation is one of the most crucial processes in proteomics research. The results
of the experiment depend on the condition of the starting material. Therefore, the proper …