We recently developed adeno-associated virus (AAV) capsids to facilitate efficient and
noninvasive gene transfer to the central and peripheral nervous systems. However, a …

Ongoing development of recombinant vectors based on adeno-associated virus (rAAV) is
providing an increasingly powerful and widely used toolkit for gene transfer and genome …

Recombinant adeno-associated virus (rAAV) shows great promise for gene therapy,
however scalability, yield and quality remain significant issues. Here we describe an rAAV …

DNA referred to as blueprint of life codes for the diversity and function of all the living
organisms. Determining DNA sequences of the living organisms not only gives an overview …

Ornithine transcarbamylase (OTC) deficiency is an X-linked urea cycle disorder associated
with high mortality. Although a promising treatment for late-onset OTC deficiency, adeno …

The chapter “Shared microbiome in different ecosystems: a meta-omics perspective”
explains recent progress in the sequencing technologies and other omics approaches that …

We recently developed novel AAV capsids for efficient and noninvasive gene transfer across
the central and peripheral nervous systems. In this protocol, we describe how to produce …

AAVs were originally discovered in 1965 as 'virus-like'particles [1, 2]. AAVs belong to a class
of small, non-enveloped, dependoparvoviruses that rely on co-infection with helper viruses …

Sequence validation of plasmid DNA is a crucial quality control step that must occur prior to
adeno-associated virus (AAV) vector packaging through plasmid transfection. AAV cis …

The use of virus-associated vectors for gene therapy and vaccination have emerged as safe
and effective delivery system. Like all other genetic materials, these vehicles are also prone …