Single-cell and spatial transcriptomics: deciphering brain complexity in health and disease

M Piwecka, N Rajewsky, A Rybak-Wolf - Nature Reviews Neurology, 2023 - nature.com
In the past decade, single-cell technologies have proliferated and improved from their
technically challenging beginnings to become common laboratory methods capable of …

Optimal transport for single-cell and spatial omics

C Bunne, G Schiebinger, A Krause, A Regev… - Nature Reviews …, 2024 - nature.com
High-throughput single-cell profiling provides an unprecedented ability to uncover the
molecular states of millions of cells. These technologies are, however, destructive to cells …

Integrated analysis of single-cell and bulk RNA sequencing data reveals a pan-cancer stemness signature predicting immunotherapy response

Z Zhang, ZX Wang, YX Chen, HX Wu, L Yin, Q Zhao… - Genome Medicine, 2022 - Springer
Background Although immune checkpoint inhibitor (ICI) is regarded as a breakthrough in
cancer therapy, only a limited fraction of patients benefit from it. Cancer stemness can be the …

Dietary restriction of cysteine and methionine sensitizes gliomas to ferroptosis and induces alterations in energetic metabolism

PS Upadhyayula, DM Higgins, A Mela, M Banu… - Nature …, 2023 - nature.com
Ferroptosis is mediated by lipid peroxidation of phospholipids containing polyunsaturated
fatty acyl moieties. Glutathione, the key cellular antioxidant capable of inhibiting lipid …

Fully-automated and ultra-fast cell-type identification using specific marker combinations from single-cell transcriptomic data

A Ianevski, AK Giri, T Aittokallio - Nature communications, 2022 - nature.com
Identification of cell populations often relies on manual annotation of cell clusters using
established marker genes. However, the selection of marker genes is a time-consuming …

Learning single-cell perturbation responses using neural optimal transport

C Bunne, SG Stark, G Gut, JS Del Castillo… - Nature …, 2023 - nature.com
Understanding and predicting molecular responses in single cells upon chemical, genetic or
mechanical perturbations is a core question in biology. Obtaining single-cell measurements …

[HTML][HTML] TGFBI secreted by tumor-associated macrophages promotes glioblastoma stem cell-driven tumor growth via integrin αvβ5-Src-Stat3 signaling

P Peng, H Zhu, D Liu, Z Chen, X Zhang, Z Guo… - Theranostics, 2022 - ncbi.nlm.nih.gov
Rationale: In the glioblastoma (GBM) microenvironment, tumor-associated macrophages
(TAMs) are prominent components and facilitate tumor growth. The exact molecular …

[HTML][HTML] Machine learning for perturbational single-cell omics

Y Ji, M Lotfollahi, FA Wolf, FJ Theis - Cell Systems, 2021 - cell.com
Cell biology is fundamentally limited in its ability to collect complete data on cellular
phenotypes and the wide range of responses to perturbation. Areas such as computer vision …

scPerturb: harmonized single-cell perturbation data

S Peidli, TD Green, C Shen, T Gross, J Min, S Garda… - Nature …, 2024 - nature.com
Abstract Analysis across a growing number of single-cell perturbation datasets is hampered
by poor data interoperability. To facilitate development and benchmarking of computational …

Single-cell analysis of 5-aminolevulinic acid intraoperative labeling specificity for glioblastoma

Z Liu, A Mela, MG Argenziano, MA Banu… - Journal of …, 2023 - thejns.org
OBJECTIVE Glioblastoma (GBM) is the most common and aggressive malignant primary
brain tumor, and resection is a key part of the standard of care. In fluorescence-guided …