Engineering synthetic biomolecular condensates
The concept of phase-separation-mediated formation of biomolecular condensates provides
a new framework to understand cellular organization and cooperativity-dependent cellular …
a new framework to understand cellular organization and cooperativity-dependent cellular …
Targeting and engineering long non-coding RNAs for cancer therapy
RNA therapeutics (RNATx) aim to treat diseases, including cancer, by targeting or
employing RNA molecules for therapeutic purposes. Amongst the most promising targets …
employing RNA molecules for therapeutic purposes. Amongst the most promising targets …
Phase separation of protein mixtures is driven by the interplay of homotypic and heterotypic interactions
Prion-like low-complexity domains (PLCDs) are involved in the formation and regulation of
distinct biomolecular condensates that form via phase separation coupled to percolation …
distinct biomolecular condensates that form via phase separation coupled to percolation …
Expanding the molecular language of protein liquid–liquid phase separation
Understanding the relationship between a polypeptide sequence and its phase separation
has important implications for analysing cellular function, treating disease and designing …
has important implications for analysing cellular function, treating disease and designing …
Interface of biomolecular condensates modulates redox reactions
Biomolecular condensates mediate diverse cellular processes. The density-transition
process of condensate formation results in the selective partitioning of molecules, which …
process of condensate formation results in the selective partitioning of molecules, which …
Programmable de novo designed coiled coil-mediated phase separation in mammalian cells
Membraneless liquid compartments based on phase-separating biopolymers have been
observed in diverse cell types and attributed to weak multivalent interactions predominantly …
observed in diverse cell types and attributed to weak multivalent interactions predominantly …
Modular RNA motifs for orthogonal phase separated compartments
JM Stewart, S Li, AA Tang, MA Klocke… - Nature …, 2024 - nature.com
Recent discoveries in biology have highlighted the importance of protein and RNA-based
condensates as an alternative to classical membrane-bound organelles. Here, we …
condensates as an alternative to classical membrane-bound organelles. Here, we …
Sequence-specific interactions determine viscoelasticity and ageing dynamics of protein condensates
Biomolecular condensates are viscoelastic materials. Here we investigate the determinants
of the sequence-encoded and age-dependent viscoelasticity of condensates formed by the …
of the sequence-encoded and age-dependent viscoelasticity of condensates formed by the …
Cross-talk of cation− π interactions with electrostatic and aromatic interactions: A salt-dependent trade-off in biomolecular condensates
Biomolecular condensates are essential for cellular functionality, yet the complex interplay
among the diverse molecular interactions that mediate their formation remains poorly …
among the diverse molecular interactions that mediate their formation remains poorly …
Designer protein compartments for microbial metabolic engineering
Highlights•Compartment formation driven by protein self-assembly or phase
transition.•Pathway enzymes can be directed into compartments for metabolic engineering.• …
transition.•Pathway enzymes can be directed into compartments for metabolic engineering.• …