Ubiquitous yet unique, lipid droplets are intracellular organelles that are increasingly being
recognized for their versatility beyond energy storage. Advances uncovering the intricacies …

Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

A main determinant of the spatial resolution of live-cell super-resolution (SR) microscopes is
the maximum photon flux that can be collected. To further increase the effective resolution …

Nonalcoholic fatty liver disease (NAFLD), recently renamed metabolic-associated fatty liver
disease (MAFLD), is one of the most common causes of liver diseases worldwide. NAFLD is …

The goal when imaging bioprocesses with optical microscopy is to acquire the most
spatiotemporal information with the least invasiveness. Deep neural networks have …

Mitochondria are traditionally known as the powerhouse of the cell, but their functions
extend far beyond energy production. They are vital in cellular and organismal pathways …

Cells contain hundreds of organelles and macromolecular assemblies. Obtaining a
complete understanding of their intricate organization requires the nanometre-level, three …

The low photostability of fluorescent proteins is a limiting factor in many applications of
fluorescence microscopy. Here we present StayGold, a green fluorescent protein (GFP) …

The performance of light microscopes is limited by the stochastic nature of light, which exists
in discrete packets of energy known as photons. Randomness in the times that photons are …

A fundamental challenge in fluorescence microscopy is the photon shot noise arising from
the inevitable stochasticity of photon detection. Noise increases measurement uncertainty …