G-protein-coupled receptors (GPCRs) are of prime importance for cell signal transduction
mechanisms and are the target of many current and potential drugs. However, structural …

Membrane proteins represent roughly one-third of the proteins encoded in the genome, yet
fewer than 1% of the proteins are of known structure. High-throughput crystallography offers …

Despite rapid progress in the field, scalable high‐yield production of adeno‐associated virus
(AAV) is still one of the critical bottlenecks the manufacturing sector is facing. The insect cell …

G protein-coupled receptors, whose topology shows seven transmembrane domains, form
the largest known family of receptors involved in higher organism signal transduction. These …

Insect cell‐based expression systems are prominent amongst current expression platforms
for their ability to express virtually all types of heterologous recombinant proteins. Stably …

The Sf9 cell/baculovirus expression system is widely used for high-level protein expression,
often with the purpose of purification. However, proteins may also be functionally expressed …

Abstract Flaviviruses such as West Nile virus (WNV), dengue virus and Zika virus are
mosquito-borne pathogens that cause significant human diseases. A novel group of insect …

The continued development of new cell culture technology is essential for the future growth
and application of insect cell and baculovirus biotechnology. The use of cell lines for …

The cell lines derived from the fall armyworm (FAW), Spodoptera frugiperda, have been
widely used for production of recombinant proteins for applications in both basic research …

Signal sequences play a key role during the first steps of the intracellular transport of G
protein-coupled receptors (GPCRs). They are involved in targeting of the nascent chains to …