Field and laboratory guidelines for reliable bioinformatic and statistical analysis of bacterial shotgun metagenomic data

O Aizpurua, RR Dunn, LH Hansen… - Critical Reviews in …, 2023 - Taylor & Francis
Shotgun metagenomics is an increasingly cost-effective approach for profiling
environmental and host-associated microbial communities. However, due to the complexity …

[HTML][HTML] Importance of downstream processing of natural astaxanthin for pharmaceutical application

L Rodríguez-Sifuentes, JE Marszalek… - Frontiers in Chemical …, 2021 - frontiersin.org
Astaxanthin (ASX) is a xanthophyll pigment considered as a nutraceutical with high
antioxidant activity. Several clinical trials have shown the multiple health benefits of this …

[HTML][HTML] Introduction of a bead beating step improves fungal DNA extraction from selected patient specimens

S Scharf, A Bartels, M Kondakci, K Pfeffer… - International Journal of …, 2020 - Elsevier
In immunocompromised patients a colonisation with fungi carries the risk to develop serious
invasive fungal infection. An early detection is therefore important, but not optimal hitherto …

[HTML][HTML] Refinement of 16S rRNA gene analysis for low biomass biospecimens

R Villette, G Autaa, S Hind, JB Holm… - Scientific Reports, 2021 - nature.com
High-throughput phylogenetic 16S rRNA gene analysis has permitted to thoroughly delve
into microbial community complexity and to understand host-microbiota interactions in …

Bioprocessing of recombinant proteins from Escherichia coli inclusion bodies: insights from structure-function relationship for novel applications

K Kachhawaha, S Singh, K Joshi, P Nain… - Preparative …, 2023 - Taylor & Francis
The formation of inclusion bodies (IBs) during expression of recombinant therapeutic
proteins using E. coli is a significant hurdle in producing high-quality, safe, and efficacious …

[HTML][HTML] Evaluation of a Highly Efficient DNA Extraction Method for Bacillus anthracis Endospores

M Knüpfer, P Braun, K Baumann, A Rehn… - Microorganisms, 2020 - mdpi.com
A variety of methods have been established in order to optimize the accessibility of DNA
originating from Bacillus anthracis cells and endospores to facilitate highly sensitive …

[HTML][HTML] An innovative protocol for metaproteomic analyses of microbial pathogens in cystic fibrosis sputum

AC Graf, J Striesow, J Pané-Farré, T Sura… - Frontiers in Cellular …, 2021 - frontiersin.org
Hallmarks of cystic fibrosis (CF) are increased viscosity of mucus and impaired mucociliary
clearance within the airways due to mutations of the cystic fibrosis conductance regulator …

Heavy and wet: The consequences of violating assumptions of measuring soil microbial growth efficiency using the 18O water method

G Pold, LA Domeignoz-Horta, KM DeAngelis - Elem Sci Anth, 2020 - online.ucpress.edu
Soils store more carbon than the biosphere and atmosphere combined, and the efficiency to
which soil microorganisms allocate carbon to growth rather than respiration is increasingly …

Revisiting soil microbial biomass: Considering changes in composition with growth rate

P Čapek, M Choma, E Kaštovská, K Tahovská… - Soil Biology and …, 2023 - Elsevier
Soil microbial biomass is assumed to have stable chemical composition. Various
components of the biomass such as DNA, ATP, or chloroform-labile organic carbon are …

[HTML][HTML] Environmentally relevant concentration of bisphenol s shows slight effects on sihumix

SS Schäpe, JL Krause, RK Masanetz, S Riesbeck… - Microorganisms, 2020 - mdpi.com
Bisphenol S (BPS) is an industrial chemical used in the process of polymerization of
polycarbonate plastics and epoxy resins and thus can be found in various plastic products …