Tools and techniques for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)/COVID-19 detection

SH Safiabadi Tali, JJ LeBlanc, Z Sadiq… - Clinical microbiology …, 2021 - Am Soc Microbiol
SUMMARY The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute
respiratory disease coronavirus 2 (SARS-CoV-2), has led to millions of confirmed cases and …

Loop‐mediated isothermal amplification (LAMP): An effective molecular point‐of‐care technique for the rapid diagnosis of coronavirus SARS‐CoV‐2

M Chaouch - Reviews in medical virology, 2021 - Wiley Online Library
The novel coronavirus disease‐2019 (Covid‐19) public health emergency has caused
enormous loss around the world. This pandemic is a concrete example of the existing gap …

Molecular diagnosis of COVID-19: challenges and research needs

W Feng, AM Newbigging, C Le, B Pang… - Analytical …, 2020 - ACS Publications
Molecular diagnosis of COVID-19 primarily relies on the detection of RNA of the SARS-CoV-
2 virus, the causative infectious agent of the pandemic. Reverse transcription polymerase …

Rapid and extraction-free detection of SARS-CoV-2 from saliva by colorimetric reverse-transcription loop-mediated isothermal amplification

MA Lalli, JS Langmade, X Chen, CC Fronick… - Clinical …, 2021 - academic.oup.com
Background Rapid, reliable, and widespread testing is required to curtail the ongoing
COVID-19 pandemic. Current gold-standard nucleic acid tests are hampered by supply …

[HTML][HTML] Large-scale implementation of pooled RNA extraction and RT-PCR for SARS-CoV-2 detection

R Ben-Ami, A Klochendler, M Seidel, T Sido… - Clinical Microbiology …, 2020 - Elsevier
Introduction Testing for active SARS-CoV-2 infection is a fundamental tool in the public
health measures taken to control the COVID-19 pandemic. Because of the overwhelming …

The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis

P Subsoontorn, M Lohitnavy, C Kongkaew - Scientific reports, 2020 - nature.com
Many recent studies reported coronavirus point-of-care tests (POCTs) based on isothermal
amplification. However, the performances of these tests have not been systematically …

[HTML][HTML] Strategies and perspectives to develop SARS-CoV-2 detection methods and diagnostics

R Jalandra, AK Yadav, D Verma, N Dalal… - Biomedicine & …, 2020 - Elsevier
To develop diagnostics and detection methods, current research is focussed on targeting the
detection of coronavirus based on its RNA. Besides the RNA target, research reports are …

SARS-CoV-2 point-of-care (POC) diagnosis based on commercial pregnancy test strips and a palm-size microfluidic device

M Yang, Y Tang, L Qi, S Zhang, Y Liu, B Lu… - Analytical …, 2021 - ACS Publications
Coronavirus diseases such as the coronavirus disease 2019 (COVID-19) pandemic, caused
by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), pose serious threats …

Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples

G Papadakis, AK Pantazis, N Fikas… - Scientific reports, 2022 - nature.com
Loop-mediated isothermal amplification is known for its high sensitivity, specificity and
tolerance to inhibiting-substances. In this work, we developed a device for performing real …

INSIGHT: A population-scale COVID-19 testing strategy combining point-of-care diagnosis with centralized high-throughput sequencing

Q Wu, C Suo, T Brown, T Wang, SA Teichmann… - Science …, 2021 - science.org
We present INSIGHT [isothermal NASBA (nucleic acid sequence–based amplification)
sequencing–based high-throughput test], a two-stage coronavirus disease 2019 testing …