The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …

The choice of biosensing elements is crucial for the development of the optimal biosensor.
Three of the most versatile biosensing elements are antibody single-chain Fv fragments …

Background Disulfide bond formation is a key posttranslational modification, with
implications for structure, function and stability of numerous proteins. While disulfide bond …

Controlling the global COVID-19 pandemic depends, among other measures, on developing
preventive vaccines at an unprecedented pace. Vaccines approved for use and those in …

Solution-phase hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) is
a widespread tool for structural analysis across academia and the biopharmaceutical …

Glutathione (GSH) is a thiol-containing tripeptide, which plays central roles in the defence
against oxidative damage and in signaling pathways. Upon oxidation, GSH is transformed to …

Revised and updated, Chemical Reagents for Protein Modification, Third Edition is an
encyclopedic work describing the many approaches to the site-specific modification of …

The majority of the thiols (SH) 2 and disulfides (SS) in cells are found as the amino acid
cysteine and its disulfide, cystine (Fig. 1 A). The thiolate anion is intrinsically one of the …

Disulfide bonds play an important role in protein folding and stability. However, the cross-
linking of sites within proteins by cysteine disulfides has significant distance and dihedral …

Asthma is an intractable disease involving the infiltration of inflammatory cells and mucus
plugging. Despite small molecular mucolytics having the ability to break the disulfide bonds …