Transposon insertion sequencing (TIS) is a powerful approach that can be extensively
applied to the genome-wide definition of loci that are required for bacterial growth under …

Epigenetics is a change in gene expression that is heritable without a change in DNA
sequence itself. This phenomenon is well studied in eukaryotes, particularly in humans for …

For decades, identifying the regions of a bacterial chromosome that are necessary for
viability has relied on mapping integration sites in libraries of random transposon mutants to …

Essential genes refer to genes that are required by an organism to survive under specific
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …

Defining the essential genome of bacterial pathogens is central to developing an
understanding of the biological processes controlling disease. This has proven elusive for …

The fast-growing Gram-negative bacterium Vibrio natriegens is an attractive microbial
system for molecular biology and biotechnology due to its remarkably short generation time …

Analysis of genes required for host infection will provide clues to the drivers of evolutionary
fitness of pathogens like Vibrio cholerae, a mounting threat to global heath. We used …

Synechococcus elongatus PCC 7942 is a model organism used for studying photosynthesis
and the circadian clock, and it is being developed for the production of fuel, industrial …

Outer membrane vesicles (OMVs) produced by Gram-negative bacteria provide an
interesting research material for defining cell-envelope proteins without experimental cell …

Transposon-insertion sequencing (TIS) is a powerful approach for deciphering genetic
requirements for bacterial growth in different conditions, as it enables simultaneous genome …