The nuclear pore complex as a flexible and dynamic gate

KE Knockenhauer, TU Schwartz - Cell, 2016 - cell.com
Nuclear pore complexes (NPCs) perforate the nuclear envelope and serve as the primary
transport gates for molecular exchange between nucleus and cytoplasm. Stripping the …

Mechanisms and functions of nuclear envelope remodelling

R Ungricht, U Kutay - Nature Reviews molecular cell biology, 2017 - nature.com
As a compartment border, the nuclear envelope (NE) needs to serve as both a protective
membrane shell for the genome and a versatile communication interface between the …

Identification of host proteins required for HIV infection through a functional genomic screen

AL Brass, DM Dykxhoorn, Y Benita, N Yan… - science, 2008 - science.org
HIV-1 exploits multiple host proteins during infection. We performed a large-scale small
interfering RNA screen to identify host factors required by HIV-1 and identified more than …

Age-dependent deterioration of nuclear pore complexes causes a loss of nuclear integrity in postmitotic cells

MA D'Angelo, M Raices, SH Panowski, MW Hetzer - Cell, 2009 - cell.com
In dividing cells, nuclear pore complexes (NPCs) disassemble during mitosis and
reassemble into the newly forming nuclei. However, the fate of nuclear pores in postmitotic …

The structure of the nuclear pore complex

A Hoelz, EW Debler, G Blobel - Annual review of biochemistry, 2011 - annualreviews.org
In eukaryotic cells, the spatial segregation of replication and transcription in the nucleus and
translation in the cytoplasm imposes the requirement of transporting thousands of …

Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation

T Pleiner, M Bates, S Trakhanov, CT Lee, JE Schliep… - elife, 2015 - elifesciences.org
Nanobodies are single-domain antibodies of camelid origin. We generated nanobodies
against the vertebrate nuclear pore complex (NPC) and used them in STORM imaging to …

Orchestrating nuclear envelope disassembly and reassembly during mitosis

S Güttinger, E Laurell, U Kutay - Nature reviews Molecular cell biology, 2009 - nature.com
Cell division in eukaryotes requires extensive architectural changes of the nuclear envelope
(NE) to ensure that segregated DNA is finally enclosed in a single cell nucleus in each …

A general amphipathic α-helical motif for sensing membrane curvature

G Drin, JF Casella, R Gautier, T Boehmer… - Nature structural & …, 2007 - nature.com
Abstract The Golgi-associated protein ArfGAP1 has an unusual membrane-adsorbing
amphipathic α-helix: its polar face is weakly charged, containing mainly serine and …

The nuclear envelope

MW Hetzer - Cold Spring Harbor perspectives in biology, 2010 - cshperspectives.cshlp.org
The nuclear envelope (NE) is a highly regulated membrane barrier that separates the
nucleus from the cytoplasm in eukaryotic cells. It contains a large number of different …

Mapping the dynamic organization of the nuclear pore complex inside single living cells

G Rabut, V Doye, J Ellenberg - Nature cell biology, 2004 - nature.com
Most cellular activities are executed by multi-protein complexes that form the basic functional
modules of their molecular machinery. Proteomic approaches can provide an evermore …