Protein engineering with bacterial display

PS Daugherty - Current opinion in structural biology, 2007 - Elsevier
Recent improvements in bacterial surface display systems coupled with efficient selection
and screening strategies are propelling bacterial display systems to the forefront of peptide …

Engineered microbial biosensors based on bacterial two-component systems as synthetic biotechnology platforms in bioremediation and biorefinery

S Ravikumar, MG Baylon, SJ Park, J Choi - Microbial cell factories, 2017 - Springer
Two-component regulatory systems (TCRSs) mediate cellular response by coupling sensing
and regulatory mechanisms. TCRSs are comprised of a histidine kinase (HK), which serves …

Staphylococcus aureus adhesion to titanium oxide surfaces coated with non-functionalized and peptide-functionalized poly (L-lysine)-grafted-poly (ethylene glycol) …

LG Harris, S Tosatti, M Wieland, M Textor, RG Richards - Biomaterials, 2004 - Elsevier
Implanted biomaterials are coated immediately with host plasma constituents, including
extracellular matrix (ECM); this reaction may be undesirable in some cases. Poly (l-lysine) …

Directed evolution of a biterminal bacterial display scaffold enhances the display of diverse peptides

JJ Rice, PS Daugherty - Protein Engineering, Design & …, 2008 - academic.oup.com
Bacterial cell-surface display systems coupled with quantitative screening methods offer the
potential to expand protein engineering capabilities. To more fully exploit this potential, a …

Reduced medical infection related bacterial strains adhesion on bioactive RGD modified titanium surfaces: a first step toward cell selective surfaces

RR Maddikeri, S Tosatti, M Schuler… - … Research Part A, 2008 - Wiley Online Library
Ideally, implants should inhibit nonspecific protein adsorption, bacterial adhesion, and at the
same time, depending on the final application be selective toward cellular adhesion and …

Construction of a bacterial biosensor for zinc and copper and its application to the development of multifunctional heavy metal adsorption bacteria

S Ravikumar, I Ganesh, I Yoo, SH Hong - Process Biochemistry, 2012 - Elsevier
In this study, we designed and applied molecular biosensors for heavy metals, zinc and
copper, for use in bioremediation strategies. Bacteria utilize two component systems to …

Biotechnological applications for surface‐engineered bacteria

H Wernérus, S Ståhl - Biotechnology and applied biochemistry, 2004 - Wiley Online Library
Display of heterologous proteins on the surface of micro‐organisms, enabled by means of
recombinant DNA technology, has become an increasingly popular strategy in microbiology …

Bacterial display using circularly permuted outer membrane protein OmpX yields high affinity peptide ligands

JJ Rice, A Schohn, PH Bessette, KT Boulware… - Protein …, 2006 - Wiley Online Library
A bacterial display methodology was developed for N‐and C‐terminal display and
demonstrated to enable rapid screening of very large peptide libraries with high precision …

The Fibrinogen Receptor FbsA Promotes Adherence of Streptococcus agalactiae to Human Epithelial Cells

A Schubert, K Zakikhany, G Pietrocola… - Infection and …, 2004 - Am Soc Microbiol
Streptococcus agalactiae is a major cause of bacterial pneumonia, sepsis, and meningitis in
human neonates. During the course of infection, S. agalactiae adheres to a variety of …

Sponge-derived fatty acids inhibit biofilm formation of MRSA and MSSA by down-regulating biofilm-related genes specific to each pathogen

NA Khan, N Barthes, G McCormack… - Journal of Applied …, 2023 - academic.oup.com
Aim A promising approach for the development of next-generation antimicrobials is to shift
their target from causing bacterial death to inhibiting virulence. Marine sponges are an …