Long-read sequencing (LRS) technologies have the potential to revolutionize scientific
discoveries in RNA biology through the comprehensive identification and quantification of …

SQANTI3 is a tool designed for the quality control, curation and annotation of long-read
transcript models obtained with third-generation sequencing technologies. Leveraging its …

Single-cell technologies have transformed biomedical research over the last decade,
opening up new possibilities for understanding cellular heterogeneity, both at the genomic …

Abstract The advancement of Long-Read Sequencing (LRS) techniques has significantly
increased the length of sequencing to several kilobases, thereby facilitating the identification …

Differential expression analysis of RNA-seq is one of the most commonly performed
bioinformatics analyses. Transcript-level quantifications are inherently more uncertain than …

Long-read RNA sequencing has emerged as a powerful tool for transcript discovery, even in
well-annotated organisms. However, assessing the accuracy of different methods in …

Accurate detection and quantification of mRNA isoforms from nanopore long-read
sequencing remains challenged by technical noise, particularly in single cells. To address …

Single-cell sequencing technologies have revolutionised the life sciences and biomedical
research. Single-cell sequencing provides high-resolution data on cell heterogeneity …

End-to-end RNA-sequencing methods that capture 5′-sequence content without
cumbersome library manipulations are of great interest, particularly for analysis of long …

In transcriptomic analyses, it is helpful to keep track of the strand of the RNA molecules.
However, the Oxford Nanopore long-read cDNA sequencing protocols generate reads that …