Membrane proteins perform some of the most important functions in the cell, including the
regulation of cell signaling through surface receptors, cell–cell interactions, and the …

Biological membranes form an essential barrier between living cells and their external
environments, as well as serve to compartmentalize intracellular organelles within …

Proteomics is the large-scale study of the structure and function of proteins in complex
biological sample. Such an approach has the potential value to understand the complex …

We have developed a new protocol for digesting hydrophobic proteins using trypsin with the
aid of phase-transfer surfactants (PTS), such as sodium deoxycholate (SDC). SDC increases …

We describe a method that allows for the concurrent proteomic analysis of both membrane
and soluble proteins from complex membrane-containing samples. When coupled with …

How one goes about analyzing proteins is a constantly evolving field that is no longer solely
the domain of the protein biochemist. Investi gators from diverse disciplines find themselves …

Integral membrane proteins (IMPs) lie at the critical junctions between intracellular
compartments and cells and their environment. As such, IMPs are in a unique position to …

▪ Abstract Mass spectrometry provides key tools for the analysis of proteins. New types of
mass spectrometers that provide enhanced capability to discover protein identities and …

The mass-spectrometry-based identification of proteins has created opportunities for the
study of organelles, transport intermediates and large subcellular structures. Traditional cell …

The proposed model is based on the measurement of the retention times of 346 tryptic
peptides in the 560-to 4,000-Da mass range, derived from a mixture of 17 protein digests …