Manipulating the destiny of wild populations using CRISPR

R Raban, JM Marshall, BA Hay… - Annual review of …, 2023 - annualreviews.org
Genetic biocontrol aims to suppress or modify populations of species to protect public
health, agriculture, and biodiversity. Advancements in genome engineering technologies …

Anopheles homing suppression drive candidates exhibit unexpected performance differences in simulations with spatial structure

SE Champer, IK Kim, AG Clark, PW Messer, J Champer - Elife, 2022 - elifesciences.org
Recent experiments have produced several Anopheles gambiae homing gene drives that
disrupt female fertility genes, thereby eventually inducing population collapse. Such drives …

The challenges in developing efficient and robust synthetic homing endonuclease gene drives

SAN Verkuijl, JXD Ang, L Alphey… - … in Bioengineering and …, 2022 - frontiersin.org
Making discrete and precise genetic changes to wild populations has been proposed as a
means of addressing some of the world's most pressing ecological and public health …

Altering traits and fates of wild populations with Mendelian DNA sequence modifying Allele Sails

ML Johnson, BA Hay, M Maselko - Nature Communications, 2024 - nature.com
Population-scale genome modification can alter the composition or fate of wild populations.
Synthetic gene drives provide one set of tools, but their use is complicated by scientific …

[PDF][PDF] A homing suppression gene drive with multiplexed gRNAs maintains high drive conversion efficiency and avoids functional resistance alleles

E Yang, M Metzloff, AM Langmüller, X Xu, AG Clark… - G3, 2022 - academic.oup.com
Gene drives are engineered alleles that can bias inheritance in their favor, allowing them to
spread throughout a population. They could potentially be used to modify or suppress pest …

Experimental demonstration of tethered gene drive systems for confined population modification or suppression

M Metzloff, E Yang, S Dhole, AG Clark, PW Messer… - BMC biology, 2022 - Springer
Background Homing gene drives hold great promise for the genetic control of natural
populations. However, current homing systems are capable of spreading uncontrollably …

Modelling homing suppression gene drive in haplodiploid organisms

Y Liu, J Champer - Proceedings of the Royal Society B, 2022 - royalsocietypublishing.org
Gene drives have shown great promise for suppression of pest populations. These
engineered alleles can function by a variety of mechanisms, but the most common is the …

Assessment of distant-site rescue elements for CRISPR toxin-antidote gene drives

J Chen, X Xu, J Champer - Frontiers in Bioengineering and …, 2023 - frontiersin.org
Gene drive is a genetic engineering technology that can enable super-mendelian
inheritance of specific alleles, allowing them to spread through a population. New gene …

The suppressive potential of a gene drive in populations of invasive social wasps is currently limited

AB Meiborg, NR Faber, BA Taylor, BA Harpur… - Scientific Reports, 2023 - nature.com
Social insects are very successful invasive species, and the continued increase of global
trade and transportation has exacerbated this problem. The yellow-legged hornet, Vespa …

Performance characteristics allow for confinement of a CRISPR toxin-antidote gene drive designed for population suppression

S Zhang, J Champer - BioRxiv, 2022 - biorxiv.org
Gene drives alleles that can bias their own inheritance are a promising way to engineer
populations for control of disease vectors, invasive species, and agricultural pests. Recent …