Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection

Y Wang, C Wang, Z Zhou, J Si, S Li, Y Zeng, Y Deng… - Biosensors, 2023 - mdpi.com
Pathogenic pathogens invade the human body through various pathways, causing damage
to host cells, tissues, and their functions, ultimately leading to the development of diseases …

Improved protocol for Bst polymerase and reverse transcriptase production and application to a point-of-care diagnostics system

LR de Souza, IEP Silva, G Celis-Silva… - Experimental …, 2023 - journals.sagepub.com
The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
has raised awareness in the scientific community about the importance of being prepared for …

Digital Microfluidic Multiplex RT-qPCR for SARS-CoV-2 Detection and Variants Discrimination

KL Ho, J Ding, JS Fan, WNT Tsui, J Bai, SK Fan - Micromachines, 2023 - mdpi.com
Continuous mutations have occurred in the genome of the SARS-CoV-2 virus since the
onset of the COVID-19 pandemic. The increased transmissibility of the mutated viruses has …

UbiNAAT: a multiplexed point-of-care nucleic acid diagnostic platform for rapid at-home pathogen detection

KP Jiang, S Bennett, EK Heiniger, S Kumar, P Yager - Lab on a Chip, 2024 - pubs.rsc.org
The COVID-19 pandemic increased demands for respiratory disease testing to facilitate
treatment and limit transmission, demonstrating in the process that most existing test options …

FlashPCR: Revolutionising qPCR by Accelerating Amplification through Low∆ T Protocols

SA Bustin, S Kirvell, T Nolan, GL Shipley - International Journal of …, 2024 - mdpi.com
Versatility, sensitivity, and accuracy have made the real-time polymerase chain reaction
(qPCR) a crucial tool for research, as well as diagnostic applications. However, for point-of …

Overview of the different methods for RNA preparation in COVID-19 diagnosis process during the pandemic

F Shahi, M Rasti, M Moradi - Analytical Biochemistry, 2023 - Elsevier
The COVID-19 pandemic brought to light the impact of a widespread disease on various
aspects of human relationships, communities, and economies. One notable consequence …

A Highly Sensitive Immunoassay for Determination of Immune Response to SARS-CoV-2 in Capillary Blood Samples

BG Sánchez, A Bort, JM Mora-Rodríguez, A Díaz-Yuste… - Biomedicines, 2022 - mdpi.com
Throughout the pandemic, serological assays have been revealed as crucial for detecting
previous exposures to the virus and determining the timing of antibody maintenance after …

External quality assessment survey for SARS-CoV-2 nucleic acid amplification tests in clinical laboratories in Tokyo, 2021

Y Sugishita, R Moriuchi, Y Ishii - Journal of Infection and Chemotherapy, 2024 - Elsevier
Introduction Nucleic acid amplification tests (NAATs) play a pivotal role in clinical
laboratories for diagnosing COVID-19. This study aimed to elucidate the accuracy of these …

[图书][B] Intelligent Real-Time Polymerase Chain Reaction System with Integrated Nucleic Acid Extraction for Point-of-Care Medical Diagnostics

T Kadja - 2023 - search.proquest.com
The recent pandemic of Corona-virus Disease 2019 (COVID-19) caused by the Severe
Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) showed an urgent need to …

[HTML][HTML] Evaluation of the AdvanSure One-Stop COVID-19 Plus Kit for SARS-CoV-2 Detection Using a Streamlined RNA Extraction Method

TY Kim, HJ Shim, E Jeong, M Kang… - Annals of laboratory …, 2023 - synapse.koreamed.org
Real-time reverse transcription (rRT)-PCR, which is the reference standard for the diagnosis
of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, generally …