How viral genetic variants and genotypes influence disease and treatment outcome of chronic hepatitis B. Time for an individualised approach?
Chronic hepatitis B virus (HBV) infection remains a global problem. Several HBV genotypes
exist with different biology and geographical prevalence. Whilst the future aim of HBV …
exist with different biology and geographical prevalence. Whilst the future aim of HBV …
[HTML][HTML] Challenges and opportunities in estimating viral genetic diversity from next-generation sequencing data
Many viruses, including the clinically relevant RNA viruses HIV (human immunodeficiency
virus) and HCV (hepatitis C virus), exist in large populations and display high genetic …
virus) and HCV (hepatitis C virus), exist in large populations and display high genetic …
[HTML][HTML] Insights from deep sequencing of the HBV genome—unique, tiny, and misunderstood
Hepatitis B virus (HBV) is a unique, tiny, partially double-stranded, reverse-transcribing DNA
virus with proteins encoded by multiple overlapping reading frames. The substitution rate is …
virus with proteins encoded by multiple overlapping reading frames. The substitution rate is …
[HTML][HTML] Quasispecies structure, cornerstone of hepatitis B virus infection: mass sequencing approach
Hepatitis B virus (HBV) is a DNA virus with complex replication, and high replication and
mutation rates, leading to a heterogeneous viral population. The population is comprised of …
mutation rates, leading to a heterogeneous viral population. The population is comprised of …
A comparative study of ultra-deep pyrosequencing and cloning to quantitatively analyze the viral quasispecies using hepatitis B virus infection as a model
In this study, the reliability and reproducibility of viral quasispecies quantification by three
ultra-deep pyrosequencing (UDPS) methods (FLX+, FLX, and Junior) were investigated and …
ultra-deep pyrosequencing (UDPS) methods (FLX+, FLX, and Junior) were investigated and …
[PDF][PDF] Characterization of the dynamics of hepatitis B virus resistance to adefovir by ultra‐deep pyrosequencing
C Rodriguez, S Chevaliez, P Bensadoun… - …, 2013 - Wiley Online Library
Hepatitis B virus (HBV) resistance to nucleoside/nucleotide analogs is frequent. Ultra‐deep
pyrosequencing (UDPS) is a powerful new tool that can detect minor viral variants and …
pyrosequencing (UDPS) is a powerful new tool that can detect minor viral variants and …
[HTML][HTML] Machine-learning based patient classification using Hepatitis B virus full-length genome quasispecies from Asian and European cohorts
AJ Mueller-Breckenridge, F Garcia-Alcalde… - Scientific Reports, 2019 - nature.com
Chronic infection with Hepatitis B virus (HBV) is a major risk factor for the development of
advanced liver disease including fibrosis, cirrhosis, and hepatocellular carcinoma (HCC) …
advanced liver disease including fibrosis, cirrhosis, and hepatocellular carcinoma (HCC) …
Deep sequencing in the management of hepatitis virus infections
The hepatitis viruses represent a major public health problem worldwide. Procedures for
characterization of the genomic composition of their populations, accurate diagnosis …
characterization of the genomic composition of their populations, accurate diagnosis …
Comparison of next-generation sequencing and clone-based sequencing in analysis of hepatitis B virus reverse transcriptase quasispecies heterogeneity
We previously reported that, based on clone-based sequencing (CBS), hepatitis B virus
(HBV) heterogeneity within the reverse transcriptase (RT) region was a predictor of antiviral …
(HBV) heterogeneity within the reverse transcriptase (RT) region was a predictor of antiviral …
Implementation of next-generation sequencing for hepatitis B virus resistance testing and genotyping in a clinical microbiology laboratory
CF Lowe, L Merrick, PR Harrigan… - Journal of clinical …, 2016 - Am Soc Microbiol
Sanger sequencing or DNA hybridization have been the primary modalities for hepatitis B
(HBV) resistance testing and genotyping; however, there are limitations, such as low …
(HBV) resistance testing and genotyping; however, there are limitations, such as low …