Publisher Summary Escherichia coli is a universal host organism both for molecular cloning
of DNA and for a diverse set of assays involving clones genes. This chapter discusses the …

Nucleic acid separation is an increasingly important tool for molecular biology. Before
modern technologies could be used, nucleic acid separation had been a time-and work …

We have used the Escherichia coli beta‐glucuronidase gene (GUS) as a gene fusion marker
for analysis of gene expression in transformed plants. Higher plants tested lack intrinsic beta …

We have developed a new vector strategy for the insertion of foreign genes into the
genomes of gram negative bacteria not closely related to Escherichia coli. The system …

A new method has been developed that makes it possible to site-specifically incorporate
unnatural amino acids into proteins. Synthetic amino acids were incorporated into the …

A modified polymerase chain reaction (PCR) was developed to introduce random point
mutations into cloned genes. The modifications were made to decrease the fidelity of Taq …

The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to
function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre …

The construction and purification of recombinant baculovirus vectors for the expression of
foreign genes in insect cells by standard transfection and plaque assay methods can take as …

[2] NEW M13 VECTORS FOR CLONING 21 plasmid vectors as a second vehicle for
recombinant DNA techniques, a A particularly important feature of this group of phage is that …

Foreign DNA fragments can be inserted into a minor coat protein gene of filamentous phage,
creating a fusion protein that is incorporated into the virion; we call these particles “fusion …