Current trends in biopharmaceuticals production in Escherichia coli

L McElwain, K Phair, C Kealey, D Brady - Biotechnology letters, 2022 - Springer
Since the manufacture of the first biotech product for a fledgling biopharmaceutical industry
in 1982, Escherichia coli, has played an important role in the industrial production of …

A unifying mechanism for protein transport through the core bacterial Sec machinery

WJ Allen, I Collinson - Open Biology, 2023 - royalsocietypublishing.org
Encapsulation and compartmentalization are fundamental to the evolution of cellular life, but
they also pose a challenge: how to partition the molecules that perform biological functions …

TerC proteins function during protein secretion to metalate exoenzymes

B He, AJ Sachla, JD Helmann - Nature Communications, 2023 - nature.com
Cytosolic metalloenzymes acquire metals from buffered intracellular pools. How exported
metalloenzymes are appropriately metalated is less clear. We provide evidence that TerC …

Strategies to Enhance Periplasmic Recombinant Protein Production Yields in Escherichia coli

A Karyolaimos, JW de Gier - Frontiers in Bioengineering and …, 2021 - frontiersin.org
Main reasons to produce recombinant proteins in the periplasm of E. coli rather than in its
cytoplasm are to-i-enable disulfide bond formation,-ii-facilitate protein isolation,-iii-control …

The biogenesis of β-lactamase enzymes

N Kaderabkova, M Bharathwaj, RCD Furniss… - …, 2022 - microbiologyresearch.org
The discovery of penicillin by Alexander Fleming marked a new era for modern medicine,
allowing not only the treatment of infectious diseases, but also the safe performance of life …

Investigation of the structure and function of SecH, a novel component of the Sec machinery in Escherichia coli

MA Wynne - 2023 - etheses.bham.ac.uk
The Sec machinery translocates proteins across, or inserts proteins into, the cytoplasmic
membrane and is responsible for translocation of approximately 20% of all proteins …

[HTML][HTML] Membrane translocation and chaperone-dependent folding of the lipase A of Pseudomonas aeruginosa

A Papadopoulos - 2023 - docserv.uni-duesseldorf.de
Beschreibungen: Die Lipase A ist ein Virulenzfaktor des opportunistischen
Humanpathogens Pseudomonas aeruginosa. Die Biogenese der Lipase ist ein komplexer …

Production and purification of endolysins from Salmonella bacteriophages

S Yetişkin - 2023 - open.metu.edu.tr
Salmonella is one of the major bacteria causing foodborne diseases in the world.
Salmonella is Gram-negative, non-spore forming and rod shape bacteria, and those with …