Networks of molecular regulators are often the primary objects of focus in the study of gene
regulation, with the machinery of protein synthesis tacitly relegated to the background …

Bacterial physiology is a branch of biology that aims to understand overarching principles of
cellular reproduction. Many important issues in bacterial physiology are inherently …

We present a whole-cell fully dynamical kinetic model (WCM) of JCVI-syn3A, a minimal cell
with a reduced genome of 493 genes that has retained few regulatory proteins or small …

INTRODUCTION The generation of biological data is presenting us with one of the most
demanding analysis challenges the world has ever faced, not only in terms of storage and …

Tight coupling of transcription and translation is considered a defining feature of bacterial
gene expression,. The pioneering ribosome can both physically associate and kinetically …

A grand challenge of systems biology is to predict the kinetic responses of living systems to
perturbations starting from the underlying molecular interactions. Changes in the nutrient …

Timely adaptation to nutrient downshift is crucial for bacteria to maintain fitness during feast
and famine cycle in the natural niche. However, the molecular mechanism that ensures the …

Protein concentrations are set by a complex interplay between gene-specific regulatory
processes and systemic factors, including cell volume and shared gene expression …

The ability to build synthetic cellular populations from the bottom-up provides the
groundwork to realize minimal living tissues comprising single cells which can communicate …

Growing cells coordinate protein translation with metabolic rates. Central to this coordination
is ribosome production. Ribosomes drive cell growth, but translation of ribosomal proteins …