Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms

L Turnbull, M Toyofuku, AL Hynen, M Kurosawa… - Nature …, 2016 - nature.com
Many bacteria produce extracellular and surface-associated components such as
membrane vesicles (MVs), extracellular DNA and moonlighting cytosolic proteins for which …

Clonal expansion under the microscope: studying lymphocyte activation and differentiation using live‐cell imaging

M Polonsky, B Chain… - Immunology and cell …, 2016 - Wiley Online Library
Clonal expansion of lymphocytes is a hallmark of vertebrate adaptive immunity. A small
number of precursor cells that recognize a specific antigen proliferate into expanded clones …

Polarized cells, polarized views: asymmetric cell division in hematopoietic cells

K Pham, F Sacirbegovic, SM Russell - Frontiers in immunology, 2014 - frontiersin.org
It has long been recognized that alterations in cell shape and polarity play important roles in
coordinating lymphocyte functions. In the last decade, a new aspect of lymphocyte polarity …

Black silicon: substrate for laser 3D micro/nano-polymerization

A Žukauskas, M Malinauskas, A Kadys… - Optics express, 2013 - opg.optica.org
We demonstrate that black silicon (b-Si) made by dry plasma etching is a promising
substrate for laser three-dimensional (3D) micro/nano-polymerization. High aspect ratio Si …

Automated profiling of individual cell–cell interactions from high-throughput time-lapse imaging microscopy in nanowell grids (TIMING)

A Merouane, N Rey-Villamizar, Y Lu, I Liadi… - …, 2015 - academic.oup.com
Motivation: There is a need for effective automated methods for profiling dynamic cell–cell
interactions with single-cell resolution from high-throughput time-lapse imaging data …

Developing T cells form an immunological synapse for passage through the β-selection checkpoint

AH Allam, M Charnley, K Pham, SM Russell - Journal of Cell Biology, 2021 - rupress.org
The β-selection checkpoint of T cell development tests whether the cell has recombined its
genomic DNA to produce a functional T cell receptor β (TCRβ). Passage through the β …

Asymmetric cell division during T cell development controls downstream fate

K Pham, R Shimoni, M Charnley… - Journal of Cell …, 2015 - rupress.org
During mammalian T cell development, the requirement for expansion of many individual T
cell clones, rather than merely expansion of the entire T cell population, suggests a possible …

The functional contribution of calcium ion flux heterogeneity in T cells

SN Christo, KR Diener… - Immunology and cell …, 2015 - Wiley Online Library
The role of intracellular calcium ion oscillations in T‐cell physiology is being increasingly
appreciated by studies that describe how unique temporal and spatial calcium ion …

A new role for Notch in the control of polarity and asymmetric cell division of developing T cells

M Charnley, M Ludford-Menting… - Journal of Cell …, 2020 - journals.biologists.com
ABSTRACT A fundamental question in biology is how single cells can reliably produce
progeny of different cell types. Notch signalling frequently facilitates fate determination …

The actin‐driven spatiotemporal organization of T‐cell signaling at the system scale

KT Roybal, P Sinai, P Verkade, RF Murphy… - Immunological …, 2013 - Wiley Online Library
T cells are activated through interaction with antigen‐presenting cells (APC s). During
activation, receptors and signaling intermediates accumulate in diverse spatiotemporal …