Molecular motors are diverse enzymes that transduce chemical energy into mechanical
work and, in doing so, perform critical cellular functions such as DNA replication and …

Cancers and developmental disorders are associated with alterations in the 3D genome
architecture in space and time (the fourth dimension). Mammalian 3D genome organization …

DNA looping is a ubiquitous and critical feature of gene regulation. Although DNA looping
can be efficiently detected, tools to readily manipulate DNA looping are limited. Here we …

The resistance of DNA to stretch, twist and bend is broadly well estimated by experiments
and is important for gene regulation and chromosome packing. However, their sequence …

Enhancers are ubiquitous and critical gene-regulatory elements. However, quantitative
understanding of the role of DNA looping in the regulation of enhancer action and specificity …

Protein-mediated DNA looping is fundamental to gene regulation and such loops occur
stochastically in purified systems. Additional proteins increase the probability of looping, but …

Abstract Tethered Particle Motion (TPM) is a versatile in vitro technique for monitoring the
conformations a linear macromolecule, such as DNA, can exhibit. The technique involves …

Supercoiling can alter the form and base pairing of the double helix and directly impact
protein binding. More indirectly, changes in protein binding and the stress of supercoiling …

DNA in bacterial chromosomes and bacterial plasmids is supercoiled. DNA supercoiling is
essential for DNA replication and gene regulation. However, the density of supercoiling in …

Protein-mediated DNA looping is ubiquitous in chromatin organization and gene regulation,
but to what extent supercoiling or nucleoid associated proteins promote looping is poorly …