When describing the structure of the DNA double helix, Watson and Crick (1) wrote,“It has
not escaped our notice that the specific pairing we have postulated immediately suggests a …

Fluorescent nucleoside analogues (FNAs) are structurally diverse mimics of the natural
essentially non-fluorescent nucleosides which have found numerous applications in probing …

Attributes contributing to the current monkeypox virus (MPXV) outbreak remain unknown. It
has been established that mutations in viral proteins may alter phenotype and pathogenicity …

The remarkable fidelity of most DNA polymerases depends on a series of early steps in the
reaction pathway which allow the selection of the correct nucleotide substrate, while …

On careful examination of existing kinetic data for correct and incorrect dNTP incorporations
by a variety of DNA polymerases, it is apparent that these enzymes resist a unified …

DNA polymerases are molecular motors directing the synthesis of DNA from nucleotides. All
polymerases have a common architectural framework consisting of three canonical …

Nanoscale pores have potential to be used as biosensors and are an established tool for
analysing the structure and composition of single DNA or RNA molecules,,. Recently …

We show that T7 DNA polymerase exists in three distinct structural states, as reported by a
conformationally sensitive fluorophore attached to the recognition (fingers) domain. The …

The present device allows for direct detection of electric charge perturbations of the local
environment by an electrode system. When used with immobilized DNA, it can uniquely …

This review summarizes our current understanding of the structural, kinetic and
thermodynamic basis for the extraordinary accuracy of high-fidelity DNA polymerases. High …