Mitochondrial DNA copy number in human disease: the more the better?

R Filograna, M Mennuni, D Alsina, NG Larsson - FEBS letters, 2021 - Wiley Online Library
Most of the genetic information has been lost or transferred to the nucleus during the
evolution of mitochondria. Nevertheless, mitochondria have retained their own genome that …

Chimeric antigen receptor natural killer (CAR-NK) cell design and engineering for cancer therapy

Y Gong, RGJ Klein Wolterink, J Wang, GMJ Bos… - Journal of hematology & …, 2021 - Springer
Due to their efficient recognition and lysis of malignant cells, natural killer (NK) cells are
considered as specialized immune cells that can be genetically modified to obtain capable …

Diagnosis of invasive fungal infections: challenges and recent developments

W Fang, J Wu, M Cheng, X Zhu, M Du, C Chen… - Journal of biomedical …, 2023 - Springer
Background The global burden of invasive fungal infections (IFIs) has shown an upsurge in
recent years due to the higher load of immunocompromised patients suffering from various …

Current and innovative methods for the diagnosis of COVID‑19 infection

L Falzone, G Gattuso, A Tsatsakis… - International …, 2021 - spandidos-publications.com
Abstract The Coronavirus Disease 2019 (COVID‑19) pandemic has forced the scientific
community to rapidly develop highly reliable diagnostic methods in order to effectively and …

Review of current COVID-19 diagnostics and opportunities for further development

Y Mardian, H Kosasih, M Karyana, A Neal… - Frontiers in …, 2021 - frontiersin.org
Diagnostic testing plays a critical role in addressing the coronavirus disease 2019 (COVID-
19) pandemic, caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV …

dPCR: a technology review

PL Quan, M Sauzade, E Brouzes - Sensors, 2018 - mdpi.com
Digital Polymerase Chain Reaction (dPCR) is a novel method for the absolute quantification
of target nucleic acids. Quantification by dPCR hinges on the fact that the random …

Droplet Cas12a assay enables DNA quantification from unamplified samples at the single-molecule level

H Yue, B Shu, T Tian, E Xiong, M Huang, D Zhu… - Nano Letters, 2021 - ACS Publications
DNA quantification is important for biomedical research, but the routinely used techniques
rely on nucleic acid amplification which have inherent issues like cross-contamination risk …

Schrödinger's microbes: tools for distinguishing the living from the dead in microbial ecosystems

JB Emerson, RI Adams, CMB Román, B Brooks… - Microbiome, 2017 - Springer
While often obvious for macroscopic organisms, determining whether a microbe is dead or
alive is fraught with complications. Fields such as microbial ecology, environmental health …

Comparison of RT-qPCR and RT-dPCR platforms for the trace detection of SARS-CoV-2 RNA in wastewater

W Ahmed, WJM Smith, S Metcalfe, G Jackson… - Acs Es&T …, 2022 - ACS Publications
We compared reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and
RT digital PCR (RT-dPCR) platforms for the trace detection of SARS-CoV-2 RNA in low …

[HTML][HTML] Absolute quantification by droplet digital PCR versus analog real-time PCR

CM Hindson, JR Chevillet, HA Briggs, EN Gallichotte… - Nature …, 2013 - nature.com
Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly
precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by …