Phage display—A powerful technique for immunotherapy: 1. Introduction and potential of therapeutic applications

J Bazan, I Całkosiński, A Gamian - Human vaccines & …, 2012 - Taylor & Francis
One of the most effective molecular diversity techniques is phage display. This technology is
based on a direct linkage between phage phenotype and its encapsulated genotype, which …

Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs

A De Marco - Microbial cell factories, 2015 - Springer
Background Antibodies have been a pillar of basic research, while their relevance in clinical
diagnostics and therapy is constantly growing. Consequently, the production of both …

High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli

K Zarschler, S Witecy, F Kapplusch, C Foerster… - Microbial cell …, 2013 - Springer
Background For their application in the area of diagnosis and therapy, single-domain
antibodies (sdAbs) offer multiple advantages over conventional antibodies and fragments …

[HTML][HTML] The improvement of anti-HER2 scFv soluble expression in Escherichia coli

F Farshdari, M Ahmadzadeh, L Nematollahi… - Brazilian Journal of …, 2020 - SciELO Brasil
The relationship between the expression of HER2 and malignity of breast tumors has led to
the generation of antibodies targeting HER2+ tumors. In addition, the expression of scFvs …

[HTML][HTML] The effect of increasing the dose of acetamiprid and dichlorvos pesticides on the reproductive performance of laboratory mice

HS Mishani, A Jalalizand… - Advanced Biomedical …, 2022 - journals.lww.com
Background: Pesticides are widely used around the world. However, these chemicals are
being used more frequently and at increased doses in underdeveloped and developing …

Structural Analyses of An Epidermal Growth Factor Receptor-Specific Single-Chain Fragment Variable via An In Silico Approach

EO Mahgoub, S Kulkarni - JoVE (Journal of Visualized Experiments), 2023 - jove.com
Single-chain fragment variable (scFv) antibodies were previously constructed of variable
light and heavy chains joined by a (Gly4-Ser) 3 linker. The linker was created using …

[HTML][HTML] Co-expression of chaperones for improvement of soluble expression and purification of an anti-HER2 scFv in Escherichia coli

AM Estabragh, HMM Sadeghi… - Advanced Biomedical …, 2022 - journals.lww.com
Background: Single-chain fragment variable (scFv) is one of the most commonly used
antibody fragments. They offer some advantages over full-length antibodies, including better …

[HTML][HTML] Single chain fragment variables antibody binding to EGF receptor in the surface of MCF7 breast cancer cell line: application and production review

EO Mahgoub - Open Journal of Genetics, 2017 - scirp.org
In this review, single-chain fragment variable construction using phage-display technology
as a promising anticancer immunotherapy technology is described. Cloning and the specific …

[PDF][PDF] Structural Analyses of An Epidermal Growth Factor Receptor-Specific Single-Chain Fragment Variable via An

EO Mahgoub, S Kulkarni - 2023 - researchgate.net
Single-chain fragment variable (scFv) antibodies were previously constructed of variable
light and heavy chains joined by a (Gly4-Ser) 3 linker. The linker was created using …

Application of phage display technology on the diagnosis of infectious diseases: discovery of Zika-specific peptides

HCS Chá-Chá - 2021 - search.proquest.com
Zika is an infectious viral disease which had a recent worldwide outbreak. This illness is
caused by the Zika virus (ZIKV), a flavivirus transmitted by the Aedes aegypti mosquito …