Isolation and characterization of a multifunctional flavonoid glycosyltransferase from Ornithogalum caudatum with glycosidase activity

S Yuan, S Yin, M Liu, JQ Kong - Scientific Reports, 2018 - nature.com
Glycosyltransferases (GTs) are bidirectional biocatalysts catalyzing the glycosylation of
diverse molecules. However, the extensive applications of GTs in glycosides formation are …

Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq

YJ Sun, JM He, JQ Kong - BMC plant biology, 2019 - Springer
Background Flavonol synthase (FLS) is the key enzyme responsible for the biosynthesis of
flavonols, the most abundant flavonoids, which have diverse pharmaceutical effects …

[HTML][HTML] The enzymatic biosynthesis of acylated steroidal glycosides and their cytotoxic activity

M Liu, JQ Kong - Acta pharmaceutica sinica B, 2018 - Elsevier
Herein we describe the discovery and functional characterization of a steroidal
glycosyltransferase (SGT) from Ornithogalum saundersiae and a steroidal glycoside …

Functional analyses of OcRhS1 and OcUER1 involved in UDP-L-rhamnose biosynthesis in Ornithogalum caudatum

S Yin, M Liu, JQ Kong - Plant Physiology and Biochemistry, 2016 - Elsevier
Abstract UDP-L-rhamnose (UDP-Rha) is an important sugar donor for the synthesis of
rhamnose-containing compounds in plants. However, only a few enzymes and their …

cDNA isolation and functional characterization of squalene synthase gene from Ornithogalum caudatum

M Liu, LN Li, YT Pan, JQ Kong - Protein Expression and Purification, 2017 - Elsevier
As the first step of ongoing efforts to investigate the genes responsible for the biosynthesis of
steroidal saponins in the medicinal plant Ornithogalum caudatum, this investigation reported …

[HTML][HTML] Steroids hydroxylation catalyzed by the monooxygenase mutant 139-3 from Bacillus megaterium BM3

X Liu, J Kong - Acta pharmaceutica sinica B, 2017 - Elsevier
The search of new substrates with pharmaceutical and industrial potential for biocatalysts
including cytochrome P450 enzymes is always challenging. Cytochrome P450 BM3 mutant …

Biosynthesis of 7, 8-dihydroxyflavone glycosides via OcUGT1-catalyzed glycosylation and transglycosylation

S Yuan, Y Yang, JQ Kong - Journal of Asian natural products …, 2018 - Taylor & Francis
Herein, a flavonoid glycosyltransferase (GT) OcUGT1 was determined to be able to attack C-
8 position of 7, 8-dihydroxyflavone (7, 8-DHF) via both glycosylation and transglycosylation …

Synthesis of UDP-apiose in Bacteria: The marine phototroph Geminicoccus roseus and the plant pathogen Xanthomonas pisi

JA Smith, M Bar-Peled - PloS one, 2017 - journals.plos.org
The branched-chain sugar apiose was widely assumed to be synthesized only by plant
species. In plants, apiose-containing polysaccharides are found in vascularized plant cell …

Transcriptome-Wide Identification of an Aurone Glycosyltransferase with Glycosidase Activity from Ornithogalum saundersiae

S Yuan, M Liu, Y Yang, JM He, YN Wang, JQ Kong - Genes, 2018 - mdpi.com
Aurone glycosides display a variety of biological activities. However, reports about
glycosyltransferases (GTs) responsible for aurones glycosylation are limited. Here, the …

cDNA Isolation and Functional Characterization of UDP-d-glucuronic Acid 4-Epimerase Family from Ornithogalum caudatum

S Yin, YJ Sun, M Liu, LN Li, JQ Kong - Molecules, 2016 - mdpi.com
d-Galacturonic acid (GalA) is an important component of GalA-containing polysaccharides in
Ornithogalum caudatum. The incorporation of GalA into these polysaccharides from UDP-d …