Nsp15, a uridine specific endoribonuclease conserved across coronaviruses, processes
viral RNA to evade detection by host defense systems. Crystal structures of Nsp15 from …

Structure-based drug design protocols may encounter difficulties to investigate poses when
the biomolecular targets do not exhibit typical binding pockets. In this study, by providing two …

The site identification by ligand competitive saturation (SILCS) method identifies the location
and approximate affinities of small molecular fragments on a target macromolecular surface …

Solution NMR spin-relaxation experiments were used to compare μs− ms dynamics in
RNase A in the apo form and as complexed to the substrate-mimic, pTppAp. The crystal …

The applicability of a computational method, Site Identification by Ligand Competitive
Saturation (SILCS), to identify regions on a protein surface with which different types of …

Noncovalent protein-ligand complexes are readily detected by electrospray ionization mass
spectrometry (ESI-MS). Ligand binding stoichiometry can be determined easily by the ESI …

Pathogenesis-related protein-4 (PR-4) family is a group of proteins with a Barwin domain in
C-terminus and generally thought to be involved in plant defense responses. However, their …

The role of the flexible loop 1 in protein conformational motion and in the dissociation of
enzymatic product from ribonuclease A (RNase A) was investigated by creation of a chimeric …

PR4 proteins possess antifungal activity against several pathogenic fungi suggesting a
pivotal role in defence reactions against plant pathogen attack. We already showed that …

Phosphoramidates composed of an amino acid and a nucleotide analogue are critical
metabolites of prodrugs, such as remdesivir. Hydrolysis of the phosphoramidate liberates …