Despite its importance, understanding the early phases of human development has been
limited by availability of human samples. The recent emergence of stem-cell-derived embryo …

3D gastruloids, aggregates of embryonic stem cells that recapitulate key aspects of gastrula-
stage embryos, have emerged as a powerful tool to study the early stages of mammalian …

Embryonic stem (ES) cells can undergo many aspects of mammalian embryogenesis in
vitro,,,–, but their developmental potential is substantially extended by interactions with …

In vitro cultured stem cells with distinct developmental capacities can contribute to
embryonic or extraembryonic tissues after microinjection into pre-implantation mammalian …

In vitro stem cell models that replicate human gastrulation have been generated, but they
lack the essential extraembryonic cells needed for embryonic development, morphogenesis …

During embryonic development, epithelial cell blocks called somites are periodically formed
according to the segmentation clock, becoming the foundation for the segmental pattern of …

Spatiotemporal orchestration of gene expression is required for proper embryonic
development. The use of single-cell technologies has begun to provide improved resolution …

Organoids are powerful models for studying tissue development, physiology, and disease.
However, current culture systems disrupt the inductive tissue-tissue interactions needed for …

Integrated in vitro models of human organogenesis are needed to elucidate the multi-
systemic events underlying development and disease. Here we report the generation of …

The segmented body plan of vertebrates is established during somitogenesis, a well-studied
process in model organisms; however, the details of this process in humans remain largely …